FastQCFastQC Report
Wed 25 May 2016
SRR1294584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA54520.29165514293616274No Hit
ACCATGTACTCTGCGTTGATACCAC51970.27801389909010227No Hit
CCCATGTACTCTGCGTTGATACCAC50570.2705245887432456No Hit
GTACATGGTAAGCAGTGGTATCAAC47670.255011017310471No Hit
GTGGTATCAACGCAGAGTACATGGG31030.16599521433068837No Hit
GGTAAGCAGTGGTATCAACGCAGAG29340.15695454684055418No Hit
GTATCAACGCAGAGTACTTTTTTTT29140.1558846453624318No Hit
GTGGTAAGCAGTGGTATCAACGCAG28980.15502872417993388No Hit
CATGTACTCTGCGTTGATACCACTG28560.15278193107587687No Hit
CCATGTACTCTGCGTTGATACCACT22600.12089886702782976No Hit
ACTCTGCGTTGATACCACTGCTTAC22150.11849158870205437No Hit
GGTATCAACGCAGAGTACATGGGGG20670.1105743177639487No Hit
GTATCAACGCAGAGTACATGGGAAG19330.10340597786052871No Hit
GGTATCAACGCAGAGTACTTTTTTT19270.10308500741709199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT508.735986E-515.1958068
TACGACC604.1004037E-412.6638494
CCCTAGG907.3486044E-611.62719251
AAGACCG752.0790515E-411.3971595
ATTAGAC1104.980502E-711.2253753
ACCGACC600.005888497511.0802768
GGATAAC700.001474521810.872181
GTGTTAC1252.1981032E-710.65473651
GTAGCAC1106.058379E-610.3618853
CCCTTAG1201.4970228E-610.3059211
TGCTAGG750.002646423210.1340612
GTCCTAA1054.0434505E-59.9661651
AGACCGT1054.117888E-59.9496356
GCTACAC1151.0285592E-59.9113683
CTAGACT1151.0292135E-59.9108374
TAAGCCT1252.577357E-69.8778024
ACTGAGC2650.09.6765758
GTCTAGG1401.0387157E-69.5131581
TTTCGTC1401.0490057E-69.5062817
TTTAACG800.00451847969.49941216
Produced by FastQC (version 0.11.5)