FastQCFastQC Report
Wed 25 May 2016
SRR1294584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105610.5649614755225265No Hit
GGTATCAACGCAGAGTACTTTTTTT68500.3664412562569176No Hit
TATCAACGCAGAGTACTTTTTTTTT65390.34980428827211446No Hit
CCCATGTACTCTGCGTTGATACCAC55350.29609523407037064No Hit
ACCATGTACTCTGCGTTGATACCAC53580.2866266059889875No Hit
GTACATGGTAAGCAGTGGTATCAAC50840.2719689557387108No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34180.18284616261111597No Hit
GTGGTAAGCAGTGGTATCAACGCAG31620.1691514236911494No Hit
GGTAAGCAGTGGTATCAACGCAGAG31600.16904443354333715No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30500.16315997541366403No Hit
CATGTACTCTGCGTTGATACCACTG30290.16203657886163553No Hit
GTGGTATCAACGCAGAGTACATGGG29490.15775697294914598No Hit
CCATGTACTCTGCGTTGATACCACT25020.1338446749131106No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24580.1314908916612414No Hit
ACTCTGCGTTGATACCACTGCTTAC23550.12598089904891108No Hit
GGTATCAACGCAGAGTACATGGGGG21340.11415848771565872No Hit
GTATCAACGCAGAGTACATGGGAAG20570.11003936702488751No Hit
GGTATCAACGCAGAGTACATGGGAA19320.1033524827866226No Hit
GTACTCTGCGTTGATACCACTGCTT19100.10217559116068797No Hit
GAGTACATGGTAAGCAGTGGTATCA18900.10110568968256559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC400.005281458614.2492588
GTTCTAT1251.0186341E-1012.927281
CGTCGAC701.0930231E-412.21201617
TACACCG600.005866998311.0857215
AGGCCCG1303.256355E-810.9639015
CGAACCA700.001493961410.85512519
TACATCG803.7639443E-410.6898025
AGACCGT803.7639443E-410.6898026
TCGCTCG803.776061E-410.68608612
TTTTCGT1252.2461609E-710.63802216
CCCTAGT909.462319E-510.5615031
TCGACCG909.52155E-510.5550059
TCGTCGA909.53447E-510.55359416
GTGTAGT1002.3842169E-510.4558881
TAGGCCC1409.65465E-810.1810374
AACGCTA750.00264327210.1355175
GCTCGTC856.6023023E-410.05749214
TCGACTG856.6055503E-410.05695419
GTATTAC1054.080081E-59.957991
TAGACTG1151.0238706E-59.9151795
Produced by FastQC (version 0.11.5)