FastQCFastQC Report
Wed 25 May 2016
SRR1294583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568663
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC32140.20488785672894688No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31930.2035491370676812No Hit
ATTCCATTCCATTCCATTCCATTCC31340.19978797230507767No Hit
GTGGTATCAACGCAGAGTACATGGG30070.1916919057821852No Hit
GTACATGGTAAGCAGTGGTATCAAC28450.18136463982384998No Hit
ACCATGTACTCTGCGTTGATACCAC27650.17626475539998074No Hit
GAATGGAATGGAATGGAATGGAATG26070.166192483662839No Hit
GTATCAACGCAGAGTACTTTTTTTT22670.1445179748613947No Hit
GTGGTAAGCAGTGGTATCAACGCAG21540.1373143881126794No Hit
CATGTACTCTGCGTTGATACCACTG17630.11238870299101847No Hit
GGTATCAACGCAGAGTACTTTTTTT16220.10340015669394892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT500.001502804913.2961839
GTCTTAG1800.012.1536191
TCTCGTG802.8748293E-511.871592510
AGCACCG658.040682E-411.6893245
CTCGTGG907.4817854E-611.6081511
CTAGTAC1001.9338713E-611.3978193
GTCTTAC1605.329639E-1010.7004691
TCGTGGG1106.060289E-610.36132212
TAATACT2300.010.3241114
GTCTCGT750.002653905710.1304259
GTCTCGC1252.5295358E-69.8919891
GTATTAG2201.6370905E-119.5115281
GTCTAGA1606.5076165E-89.5115281
GACAGTC900.00111612639.4975777
ACAGTGC2600.09.4969718
CCCTTAC1651.04513674E-79.22331
CTTCTAG1554.183421E-79.2047041
TCTCGCT1356.838567E-69.1487322
TATACTG4050.09.1458145
GTCTAAT1252.7139542E-59.1310661
Produced by FastQC (version 0.11.5)