FastQCFastQC Report
Wed 25 May 2016
SRR1294583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568663
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79430.5063547747349175No Hit
GGTATCAACGCAGAGTACTTTTTTT59990.38242758323489495No Hit
TATCAACGCAGAGTACTTTTTTTTT45270.28858970983570087No Hit
CCCATGTACTCTGCGTTGATACCAC35160.22413992042905326No Hit
ATTCCATTCCATTCCATTCCATTCC32100.2046328625077534No Hit
ACCATGTACTCTGCGTTGATACCAC30230.19271188266695907No Hit
GTGGTATCAACGCAGAGTACATGGG29970.19105442022920155No Hit
GTACATGGTAAGCAGTGGTATCAAC29220.18627327858182413No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25590.16313255300851745No Hit
GAATGGAATGGAATGGAATGGAATG25160.16039136513068775No Hit
GTGGTAAGCAGTGGTATCAACGCAG24760.1578414229187531No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22170.14133054709647644No Hit
GGTAAGCAGTGGTATCAACGCAGAG18180.11589487353242858No Hit
CATGTACTCTGCGTTGATACCACTG18150.11570362786653347No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16070.10244392836447344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1302.6066118E-911.6943093
TCGCTCT1158.795414E-710.740974
GTCTTAG1605.366019E-1010.6954681
CTAAGGC803.7647574E-410.6893293
CTAGTAC909.497789E-510.5573623
CGCTCTG1106.0318325E-610.3654115
CTTAGAC1303.8309554E-710.2325213
TCTGTCG1054.106619E-59.9518648
TCTAGAC1356.424907E-79.8535383
TATACTG5300.09.6809025
GTGTAGG1804.125468E-99.5070821
CCTCTAG1401.0473414E-69.5070821
CTAAGGG1106.821997E-59.5016273
TCAAGAC1901.0477379E-99.5016273
TAAGACC1304.2276242E-69.5016264
GACTGAC900.00111373069.4998087
ATACTGT5750.09.41900356
GTACTAT2151.05501385E-109.2859881
GTCTAGA1451.6881495E-69.1792521
CTCGCTC1252.7474869E-59.1215613
Produced by FastQC (version 0.11.5)