FastQCFastQC Report
Wed 25 May 2016
SRR1294582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714203
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC43700.25492896698932394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41080.239644896199575No Hit
ATTCCATTCCATTCCATTCCATTCC39700.23159450776833312No Hit
ACCATGTACTCTGCGTTGATACCAC35910.2094851076564444No Hit
GTACATGGTAAGCAGTGGTATCAAC34130.19910127330310357No Hit
GAATGGAATGGAATGGAATGGAATG32900.19192592709264888No Hit
GTGGTATCAACGCAGAGTACATGGG29940.17465842726911573No Hit
GTGGTAAGCAGTGGTATCAACGCAG27530.1605994155884688No Hit
CATGTACTCTGCGTTGATACCACTG22300.13008961015702342No Hit
GGTAAGCAGTGGTATCAACGCAGAG19580.11422217788674971No Hit
GTATCAACGCAGAGTACTTTTTTTT18500.10792187389708222No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA18000.10500506649445836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCG350.002174534116.2809895
TAGGACC752.0796509E-411.3966914
GTATTAG2350.011.3330571
GAGTGCG600.005887505611.080440511
CTAGGAC1303.28273E-810.9577183
CTTACAC2500.010.2564243
TCTGTCG750.002653549710.1306888
ATAGGAC1502.4572728E-810.1298013
TAACACC1252.5791269E-69.8771324
GTCCAAG1752.5174813E-99.7834371
ATAAGAC1856.530172E-109.7533563
GCTCTAA1002.7214296E-49.5116741
GTGTAGG2201.6370905E-119.5116741
GGTCTAA1002.7214296E-49.5116741
TAGGGGT900.00111659679.4972434
GGACCTC1002.7632504E-49.4972436
TTAGGAC1106.861899E-59.4966893
GTATTGG1856.553819E-99.25460151
TAGGACA1752.6808266E-89.2258934
CCTATAC1451.7138063E-69.1692173
Produced by FastQC (version 0.11.5)