FastQCFastQC Report
Wed 25 May 2016
SRR1294582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714203
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68710.40082767326856855No Hit
GGTATCAACGCAGAGTACTTTTTTT50830.2965226405507399No Hit
CCCATGTACTCTGCGTTGATACCAC45170.263504380753038No Hit
ATTCCATTCCATTCCATTCCATTCC40640.237078105685266No Hit
TATCAACGCAGAGTACTTTTTTTTT39070.2279193304410271No Hit
ACCATGTACTCTGCGTTGATACCAC37790.22045230349031006No Hit
GTACATGGTAAGCAGTGGTATCAAC37250.2173021514954763No Hit
GAATGGAATGGAATGGAATGGAATG33450.19513441523553512No Hit
GTGGTAAGCAGTGGTATCAACGCAG31960.18644232917571607No Hit
GTGGTATCAACGCAGAGTACATGGG30820.1797920082977337No Hit
CATGTACTCTGCGTTGATACCACTG23250.1356315442220087No Hit
GGTAAGCAGTGGTATCAACGCAGAG21080.12297260009462123No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20960.12227256631799151No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20330.11859738899068548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19200.11200540426075559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGA500.001498182713.3017376
ATCCGTA550.003070361412.09001811
TACCCGA600.00587930111.08251711
CGAGTTG951.3623019E-510.99854819
GTCCTAA1408.265488E-910.8642661
TAGGGTG1252.23592E-710.6413895
GCACTGT3100.010.11422356
GTTCTAG1803.8926373E-1010.0343571
CGAATGT1151.028304E-59.91144715
CTACACT2400.09.8971254
AGGACAG2700.09.8531385
GTATAGC900.0011069759.5062331
TATTCCG1201.6975364E-59.5012415
ATTAGAC1304.2306638E-69.501243
GTACTGT1804.1709427E-99.501246
ATTTCGT800.004521869.49846816
TAAGACT2401.0913936E-119.1053554
GTTTAGG1151.1025579E-49.0929191
GGAACGA1151.1124148E-49.08549215
CCTGGAC3150.09.04883
Produced by FastQC (version 0.11.5)