FastQCFastQC Report
Wed 25 May 2016
SRR1294581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796593
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC344461.9172956813257092No Hit
ACCATGTACTCTGCGTTGATACCAC242031.3471609874913237No Hit
GTACATGGTAAGCAGTGGTATCAAC231891.2907208254735492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA210551.1719404450534985No Hit
GTGGTAAGCAGTGGTATCAACGCAG186671.0390221936743602No Hit
CATGTACTCTGCGTTGATACCACTG182531.0159785772292333No Hit
GGTAAGCAGTGGTATCAACGCAGAG146260.8140964592425776No Hit
GTATCAACGCAGAGTACATGGGAAG113220.6301928149558638No Hit
CCATGTACTCTGCGTTGATACCACT112420.625739942212844No Hit
ACTCTGCGTTGATACCACTGCTTAC105940.5896716729943844No Hit
GTACTCTGCGTTGATACCACTGCTT100740.5607280001647563No Hit
GTACATGGGAAGCAGTGGTAAGCAG100480.5592808165232749No Hit
GCAGTGGTATCAACGCAGAGTACAT92000.512080365447266No Hit
GGTATCAACGCAGAGTACATGGGAA87490.48697729535849243No Hit
GAGTACATGGTAAGCAGTGGTATCA83490.4647129316433939No Hit
GCTTACCACTGCTTCCCATGTACTC80920.4504080779564431No Hit
GTACATGGGAAGCAGTGGTATCAAC79790.4441183952069278No Hit
GTACATGATAAGCAGTGGTATCAAC78930.4393315570081816No Hit
GCTTACCATGTACTCTGCGTTGATA78760.4383853215502899No Hit
GTGGTATCAACGCAGAGTACATGGG78130.4348786842651619No Hit
GCGTTGATACCACTGCTTACCACTG76670.42675219150915095No Hit
GCGTTGATACCACTGCTTACCATGT74480.4145624523751345No Hit
TATCAACGCAGAGTACATGGGAAGC74460.414451130556559No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA74310.4136162169172428No Hit
ATCATGTACTCTGCGTTGATACCAC72700.40465481052191565No Hit
AAGCAGTGGTATCAACGCAGAGTAC69860.38884711228419566No Hit
CTGCTTACCATGTACTCTGCGTTGA68330.38033099316317054No Hit
GTATCAACGCAGAGTACATGGTAAG61690.343372149396107No Hit
CAGTGGTATCAACGCAGAGTACATG56320.3134822411085872No Hit
GTACATAAGCAGTGGTATCAACGCA53130.2957264110457961No Hit
ATGGTAAGCAGTGGTATCAACGCAG52910.2945018710414657No Hit
TATCAACGCAGAGTACATGGTAAGC51090.2843715855510959No Hit
ACGCAGAGTACATGGTAAGCAGTGG48760.271402593687051No Hit
ACGCAGAGTACATGGGAAGCAGTGG46020.2561515045422085No Hit
GTGATAAGCAGTGGTATCAACGCAG41450.23071446899770842No Hit
GGTATCAACGCAGAGTACATGGTAA40760.22687386625685393No Hit
GAGTACATGGGAAGCAGTGGTAAGC39770.22136343623736704No Hit
TACCACTGCTTACCATGTACTCTGC38520.21440582257639876No Hit
GTATCAACGCAGAGTACTTTTTTTT37240.20728122618756725No Hit
GTGGTATCAACGCAGAGTACATGGT37210.207114243459704No Hit
GAGTAAGCAGTGGTATCAACGCAGA36960.20572272072751033No Hit
GCTTCCCATGTACTCTGCGTTGATA34750.19342165977491843No Hit
GAGTACATGGGAAGCAGTGGTATCA34530.192197119770588No Hit
GTACATGGTAAGCAGTGGTAAGCAG33440.18613008065822365No Hit
AAAAAGTACTCTGCGTTGATACCAC32620.18156588609662846No Hit
GCTTATGTACTCTGCGTTGATACCA31850.177279996081472No Hit
GAGTACATGATAAGCAGTGGTATCA30150.16781764150255513No Hit
GATAAGCAGTGGTATCAACGCAGAG29990.16692706695395118No Hit
ACTCTGCGTTGATACCACTGCTTAT27940.1555165805499632No Hit
GCTTACCACTGCTTACCATGTACTC27890.15523827600352447No Hit
AAAGTACTCTGCGTTGATACCACTG27130.15100804689765573No Hit
GTAAGCAGTGGTATCAACGCAGAGT26990.1502287941676273No Hit
GCAGAGTACATGGTAAGCAGTGGTA26960.15006181143976405No Hit
CTGCTTACCACTGCTTCCCATGTAC26870.14956086325617432No Hit
GCGTTGATACCACTGCTTCCCATGT26450.147223105066089No Hit
GCTTATCATGTACTCTGCGTTGATA24900.1385956641264883No Hit
TATCAACGCAGAGTACTTTTTTTTT24630.13709281957571914No Hit
TGGTAAGCAGTGGTATCAACGCAGA24340.1354786532063745No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT24270.1350890268413603No Hit
CTTCCCATGTACTCTGCGTTGATAC23520.1309144586447793No Hit
GGTATCAACGCAGAGTACTTTTTTT23160.12891066591042044No Hit
CTGCTTCCCATGTACTCTGCGTTGA22180.1234558968002213No Hit
GCGTTGATACCACTGCTTATCATGT22030.12262098316090511No Hit
GTTGATACCACTGCTTACCATGTAC21340.11878038042005062No Hit
GCTTACTCTGCGTTGATACCACTGC20690.1151624213163471No Hit
GATACCACTGCTTACCATGTACTCT19350.1077038594717891No Hit
ATACCACTGCTTACCATGTACTCTG18830.1048094921888263No Hit
GTTGATACCACTGCTTACCACTGCT18480.10286136036375516No Hit
TACCACTGCTTACCACTGCTTCCCA18140.1009688894479718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC250.005995257819.0203461
TGGACCG400.005288873314.24620155
GAACAAA6500.013.8994841
CCGCCCT500.00150177713.297564515
CAGACTA604.1017952E-412.6632895
CGCCCTA550.003069775612.09037816
CCTACAC550.00307425912.0880223
TGTTGCA752.0728595E-411.4007682
TAAAGGT752.0782262E-411.3975954
CACAACG1604.5474735E-1111.2779289
GTATAGG600.00582963411.09520151
TCAGAAC600.00588671911.0806863
CAGTACT951.3635958E-510.997684
GTTTTAC700.001478339110.8687691
TTGCGTT700.001494226310.85485211
TTTGCGT700.001494920510.85424710
TTAACAC1502.1409505E-910.7640953
AACGTTG1158.841507E-710.73686512
TTGGACT803.778649E-410.68524554
CTATACT803.778649E-410.68524554
Produced by FastQC (version 0.11.5)