FastQCFastQC Report
Wed 25 May 2016
SRR1294581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796593
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC359622.0016776198059327No Hit
GTACATGGTAAGCAGTGGTATCAAC252401.4048813504227167No Hit
ACCATGTACTCTGCGTTGATACCAC251901.4020983049583295No Hit
GTGGTAAGCAGTGGTATCAACGCAG208801.162199785928143No Hit
CATGTACTCTGCGTTGATACCACTG193331.0760923592599994No Hit
GGTAAGCAGTGGTATCAACGCAGAG167690.9333777878462177No Hit
GTATCAACGCAGAGTACTTTTTTTT129050.7183040343583661No Hit
GTATCAACGCAGAGTACATGGGAAG123460.6871895860665159No Hit
CCATGTACTCTGCGTTGATACCACT120570.6711035832823572No Hit
GTACATGGGAAGCAGTGGTAAGCAG107090.5960726775624753No Hit
ACTCTGCGTTGATACCACTGCTTAC106900.595015120286008No Hit
GCAGTGGTATCAACGCAGAGTACAT99980.5564977710588875No Hit
GTACTCTGCGTTGATACCACTGCTT99000.5510430019486885No Hit
GGTATCAACGCAGAGTACATGGGAA93030.5178134391039039No Hit
GAGTACATGGTAAGCAGTGGTATCA92750.516254933643847No Hit
GTACATGGGAAGCAGTGGTATCAAC86350.4806319516996894No Hit
TATCAACGCAGAGTACTTTTTTTTT83980.46744031619849347No Hit
GTGGTATCAACGCAGAGTACATGGG83240.46332140891120027No Hit
TATCAACGCAGAGTACATGGGAAGC81660.4545269852437363No Hit
GCTTACCACTGCTTCCCATGTACTC81590.4541373588787221No Hit
GGTATCAACGCAGAGTACTTTTTTT81460.4534137670579814No Hit
AAGCAGTGGTATCAACGCAGAGTAC80040.4455099179391214No Hit
GCTTACCATGTACTCTGCGTTGATA79690.4435617861140503No Hit
GTACTTTTTTTTTTTTTTTTTTTTT78540.4371607815459595No Hit
GTACATGATAAGCAGTGGTATCAAC78100.4347117015372987No Hit
GCGTTGATACCACTGCTTACCACTG77650.43220696061935004No Hit
ATCATGTACTCTGCGTTGATACCAC75740.4215757269453905No Hit
GCGTTGATACCACTGCTTACCATGT75190.4185143769345645No Hit
CTGCTTACCATGTACTCTGCGTTGA69930.3892367386492099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA68970.38389329135758626No Hit
GTATCAACGCAGAGTACATGGTAAG66570.37053467312852717No Hit
CAGTGGTATCAACGCAGAGTACATG62960.35044108487565073No Hit
ATGGTAAGCAGTGGTATCAACGCAG60040.3341880993636288No Hit
TATCAACGCAGAGTACATGGTAAGC55430.3085284201819778No Hit
GTACATAAGCAGTGGTATCAACGCA54080.301014197428132No Hit
ACGCAGAGTACATGGTAAGCAGTGG53940.3002349446981036No Hit
ACGCAGAGTACATGGGAAGCAGTGG52470.29205279103280485No Hit
GTGATAAGCAGTGGTATCAACGCAG51460.2864310391947425No Hit
GAGTACTTTTTTTTTTTTTTTTTTT48340.26906483549696564No Hit
GAGTACATGGGAAGCAGTGGTAAGC45510.25331279816853347No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43780.24368346086175333No Hit
GGTATCAACGCAGAGTACATGGTAA43000.23934190993730914No Hit
GAGTAAGCAGTGGTATCAACGCAGA39840.22175306260238128No Hit
GTGGTATCAACGCAGAGTACATGGT38960.2168549025850596No Hit
TACCACTGCTTACCATGTACTCTGC38390.21368223075565804No Hit
GTACATGGTAAGCAGTGGTAAGCAG37430.20833878346403442No Hit
GAGTACATGGGAAGCAGTGGTATCA37380.20806047891759571No Hit
GCTTCCCATGTACTCTGCGTTGATA36520.2032736407188495No Hit
GATAAGCAGTGGTATCAACGCAGAG34710.1931990161377674No Hit
GTGGTATCAACGCAGAGTACTTTTT33860.188467838848309No Hit
AAAAAGTACTCTGCGTTGATACCAC33520.1865753679325256No Hit
GCTTATGTACTCTGCGTTGATACCA32010.1781705706300759No Hit
GAGTACATGATAAGCAGTGGTATCA31110.17316108879417874No Hit
GCAGAGTACATGGTAAGCAGTGGTA29820.1659808314960595No Hit
GTAAGCAGTGGTATCAACGCAGAGT29730.16547988331246977No Hit
ACTCTGCGTTGATACCACTGCTTAT28700.1597468096558319No Hit
AAAGTACTCTGCGTTGATACCACTG27720.15429204054563275No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT27450.15278919599486362No Hit
TGGTAAGCAGTGGTATCAACGCAGA27160.151175029625519No Hit
CTGCTTACCACTGCTTCCCATGTAC27010.15034011598620278No Hit
GCTTACCACTGCTTACCATGTACTC26950.15000615053047628No Hit
GCGTTGATACCACTGCTTCCCATGT26710.14867028870757037No Hit
CTTCCCATGTACTCTGCGTTGATAC24790.1379833941243231No Hit
GCTTATCATGTACTCTGCGTTGATA24550.13664753230141718No Hit
GCGTTGATACCACTGCTTATCATGT22700.12635026408318412No Hit
CTGCTTCCCATGTACTCTGCGTTGA22440.1249030804417027No Hit
GCAGTGGTATCAACGCAGAGTACTT21380.1190030240572016No Hit
GTTGATACCACTGCTTACCATGTAC20820.11588601313708782No Hit
GCAGTGGTAAGCAGTGGTATCAACG20790.11571903040922457No Hit
GCTTACTCTGCGTTGATACCACTGC20460.11388222040272895No Hit
GATACCACTGCTTACCATGTACTCT20340.11321428949127599No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT20270.11282466312626176No Hit
ATACCACTGCTTACCATGTACTCTG19260.10720291128819938No Hit
CATGGTAAGCAGTGGTATCAACGCA19210.10692460674176064No Hit
CTTATGTACTCTGCGTTGATACCAC19140.10653498037674641No Hit
CCACTGCTTACCATGTACTCTGCGT18930.10536610128170376No Hit
GTTGATACCACTGCTTACCACTGCT18810.1046981703702508No Hit
GTACATGGGAAGCAGTGATAAGCAG18600.10352929127520813No Hit
ATCAACGCAGAGTACATGGGAAGCA18490.10291702127304292No Hit
GCAGTAAGCAGTGGTATCAACGCAG18190.10124719399441053No Hit
AAGCAGTGGTAAGCAGTGGTATCAA18140.1009688894479718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG550.003048582712.1015661
AGCGATG550.003071560812.08943912
GCGTTGG1406.82121E-1011.5339913
TAGACTG855.312189E-511.1788455
GCTCTAA600.005837856311.09310151
GCTAAAT600.005837856311.09310151
GAATAAG803.7407136E-410.6969191
GTGCTAT909.431431E-510.5648581
TTAATCA1201.5195747E-610.2938534
CGATGAT1303.849218E-710.22923914
CGTTGGT1409.709038E-810.17705114
ACATGAC951.6406251E-410.0021253
TTACATC951.6406251E-410.0021254
GTCATAG1151.0157322E-59.9217821
GAACTGT1151.0238347E-59.9151516
GTACTGT1451.6118793E-79.8296756
TCACTCT1451.6118793E-79.8296754
TGCGTTA1554.0701707E-89.80525912
AAAAGTA7550.09.6910712
GAACAAA3350.09.650291
Produced by FastQC (version 0.11.5)