FastQCFastQC Report
Wed 25 May 2016
SRR1294580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501803
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC131210.5244617581800006No Hit
GTACATGGTAAGCAGTGGTATCAAC112140.4482367316691202No Hit
ACCATGTACTCTGCGTTGATACCAC108310.43292777249047987No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA81560.32600488527673843No Hit
GTGGTAAGCAGTGGTATCAACGCAG77000.30777803048441466No Hit
CATGTACTCTGCGTTGATACCACTG65840.26317020165056965No Hit
GGTAAGCAGTGGTATCAACGCAGAG59190.23658937174509745No Hit
CCATGTACTCTGCGTTGATACCACT47810.19110217710986835No Hit
GTGGTATCAACGCAGAGTACATGGG45540.18202872088649666No Hit
GTATCAACGCAGAGTACATGGGAAG41820.1671594446085483No Hit
ACTCTGCGTTGATACCACTGCTTAC41530.16600028059763297No Hit
GAGTACATGGTAAGCAGTGGTATCA41040.164041693130914No Hit
GCTTACCATGTACTCTGCGTTGATA38470.1537691017238368No Hit
GCAGTGGTATCAACGCAGAGTACAT38270.1529696782680331No Hit
GTACTCTGCGTTGATACCACTGCTT34890.139459421864951No Hit
GTACATGGGAAGCAGTGGTAAGCAG34530.13802045964450438No Hit
GCGTTGATACCACTGCTTACCATGT33440.13366360181037434No Hit
GGTATCAACGCAGAGTACATGGGAA32690.1306657638511106No Hit
GCTTACCACTGCTTCCCATGTACTC29250.11691568041128737No Hit
GTACATGGGAAGCAGTGGTATCAAC28270.11299850547784938No Hit
GTATCAACGCAGAGTACATGGTAAG27220.10880153233488007No Hit
CTGCTTACCATGTACTCTGCGTTGA27180.10864164764371935No Hit
GTACATGATAAGCAGTGGTATCAAC26600.10632331962188868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC1950.012.1772583
CGTAGCA658.019904E-411.693442
CTAGGAC1900.011.4978943
AGCGTGC600.005887998311.080648
TAGGACC1158.845891E-710.736954
CGAATGT750.002651243510.13208515
CAGTCCG750.002653882610.130879
GTCCTAA1451.5878322E-79.8403431
GTACACG1553.9854058E-89.8191811
CTAGACA1401.0620424E-69.4980714
GTCTAGT1651.04513674E-79.2240791
GCGAAAA1054.4359878E-49.0593631
GTGCTAA2850.09.01168251
GTATAAA4650.09.0009161
ACTGACC1901.0628355E-88.9978138
TCAATAC2502.5465852E-118.73843
CTAGTAC1201.7678253E-48.7067383
GTATTAA5050.08.6646981
GCTTATG4400.08.6475741
TAGACAG2751.8189894E-128.6344385
Produced by FastQC (version 0.11.5)