FastQCFastQC Report
Wed 25 May 2016
SRR1294580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501803
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC136350.545006940994155No Hit
GTACATGGTAAGCAGTGGTATCAAC114520.4577498707931839No Hit
ACCATGTACTCTGCGTTGATACCAC114270.4567505914734294No Hit
GTGGTAAGCAGTGGTATCAACGCAG90950.3635378165267209No Hit
GTATCAACGCAGAGTACTTTTTTTT84700.3385558335328561No Hit
CATGTACTCTGCGTTGATACCACTG70720.2826761339721792No Hit
GGTAAGCAGTGGTATCAACGCAGAG69130.27632071749854004No Hit
GGTATCAACGCAGAGTACTTTTTTT57920.2315130328007441No Hit
CCATGTACTCTGCGTTGATACCACT51180.2045724623401603No Hit
TATCAACGCAGAGTACTTTTTTTTT50500.20185442259042777No Hit
GTGGTATCAACGCAGAGTACATGGG47070.18814431032339476No Hit
GTATCAACGCAGAGTACATGGGAAG45280.18098947039395188No Hit
ACTCTGCGTTGATACCACTGCTTAC43510.17391457281008937No Hit
GAGTACATGGTAAGCAGTGGTATCA43340.17323506287265625No Hit
GCAGTGGTATCAACGCAGAGTACAT41440.16564054004252132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40170.160564201098168No Hit
GCTTACCATGTACTCTGCGTTGATA39890.15944500826004285No Hit
GTACATGGGAAGCAGTGGTAAGCAG37740.15085120611015335No Hit
GTACTCTGCGTTGATACCACTGCTT34820.1391796236554197No Hit
GGTATCAACGCAGAGTACATGGGAA34480.13782060378055347No Hit
GCGTTGATACCACTGCTTACCATGT34060.13614181452336574No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32570.13018610977762837No Hit
GCTTACCACTGCTTCCCATGTACTC31590.12626893484419038No Hit
GTACATGGGAAGCAGTGGTATCAAC30160.1205530571351941No Hit
GTACATGATAAGCAGTGGTATCAAC29910.11955377781543951No Hit
AAGCAGTGGTATCAACGCAGAGTAC29470.11779504621267142No Hit
CTGCTTACCATGTACTCTGCGTTGA28400.11351813072412176No Hit
GTATCAACGCAGAGTACATGGTAAG27360.10936112875394265No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27220.10880153233488007No Hit
ATGGTAAGCAGTGGTATCAACGCAG26320.10520412678376354No Hit
CAGTGGTATCAACGCAGAGTACATG26000.10392504925447767No Hit
TATCAACGCAGAGTACATGGGAAGC25830.10324553931704454No Hit
GCGTTGATACCACTGCTTACCACTG25630.10244611586124087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC400.005284939414.24811110
GCACCGT604.0850462E-412.6697996
GTCTACG752.0608112E-411.4082941
ACCGTGA700.001492856410.8563568
TTTGCGT700.001493603510.85570310
AGCACCG1002.3939645E-510.4523755
GTCTTAC1502.419074E-810.1407061
CTGTGCG856.600342E-410.0580939
GTCTTAT2201.6552804E-109.0747791
GTCTTAG2201.6552804E-109.0747791
GAACAGT3150.09.0498566
TAGACAG3100.08.8891175
GTACTGG3550.08.8374111
TTATACT4650.08.7869434
GTATTAA4550.08.7756111
GTCTATG1651.0394306E-68.6426471
TGGACTA1651.0469303E-68.6383275
TAGAACC1107.1300304E-48.6383274
TCGCTCT1107.1300304E-48.6383274
CTACACT3200.08.6113324
Produced by FastQC (version 0.11.5)