FastQCFastQC Report
Wed 25 May 2016
SRR1294579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627642
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC101490.3862398302356257No Hit
ATTCCATTCCATTCCATTCCATTCC84380.32112441496977134No Hit
ACCATGTACTCTGCGTTGATACCAC80740.307271690740215No Hit
GTACATGGTAAGCAGTGGTATCAAC77790.29604489500472286No Hit
GAATGGAATGGAATGGAATGGAATG69180.2632778742309645No Hit
GTGGTAAGCAGTGGTATCAACGCAG65580.24957737774019442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA54440.20718195248820045No Hit
CATGTACTCTGCGTTGATACCACTG50670.1928344881075885No Hit
GTGGTATCAACGCAGAGTACATGGG44460.16920113166101014No Hit
GGTAAGCAGTGGTATCAACGCAGAG42660.16235088341562512No Hit
CCATGTACTCTGCGTTGATACCACT34300.13053528600928133No Hit
ACTCTGCGTTGATACCACTGCTTAC33600.12787130058052049No Hit
GTATCAACGCAGAGTACATGGGAAG30980.1179003836900156No Hit
GAGTACATGGTAAGCAGTGGTATCA29000.11036511062009209No Hit
GTACATGGGAAGCAGTGGTAAGCAG28600.10884283323222875No Hit
GCTTACCATGTACTCTGCGTTGATA28200.1073205558443654No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA27990.10652136021573715No Hit
GGTATCAACGCAGAGTACATGGGAA27610.10507519669726699No Hit
GCTTACCACTGCTTCCCATGTACTC26720.10168812950927106No Hit
GCAGTGGTATCAACGCAGAGTACAT26420.10054642146837353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTA508.7369765E-515.19604113
GTCGGCT400.00529018714.24601811
AGCACCG604.102703E-412.6633675
CCGTCCA802.8774613E-511.871239
CAGGACG658.0352306E-411.690824
TCGGAAC700.001495241510.85431513
CGAAATG1502.1173037E-910.77226819
GTTAGAC1201.5268506E-610.2905533
CGCACAG750.002654340810.1306945
GCACCGT856.613141E-410.0560136
GGAACGA2750.010.01690815
ACGAAAT1651.0142685E-89.79297218
TATTCCG2251.8189894E-129.7085825
TCCTATA1304.229267E-69.5020472
GAACGAT800.00452052439.49915316
AAATGCG800.00452691649.4975265
GACCGTG800.0045276279.4973457
GCTACAC2151.07320375E-109.2780673
GTATTAG3400.09.23417951
GTGTAGG2501.8189894E-129.1334431
Produced by FastQC (version 0.11.5)