FastQCFastQC Report
Wed 25 May 2016
SRR1294579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627642
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC106790.40641000562481494No Hit
GTATCAACGCAGAGTACTTTTTTTT88310.3360807903055287No Hit
ATTCCATTCCATTCCATTCCATTCC86750.3301439084928616No Hit
ACCATGTACTCTGCGTTGATACCAC84250.3206296748187158No Hit
GTACATGGTAAGCAGTGGTATCAAC81400.3097834484301895No Hit
GTGGTAAGCAGTGGTATCAACGCAG76360.2906027533431114No Hit
GAATGGAATGGAATGGAATGGAATG67770.2579118464387462No Hit
GGTATCAACGCAGAGTACTTTTTTT61420.23374569290641573No Hit
TATCAACGCAGAGTACTTTTTTTTT53200.2024628925858241No Hit
CATGTACTCTGCGTTGATACCACTG52680.20048393198160175No Hit
GGTAAGCAGTGGTATCAACGCAGAG49560.1886101683562677No Hit
GTGGTATCAACGCAGAGTACATGGG46880.17841090985758334No Hit
CCATGTACTCTGCGTTGATACCACT38120.1450730350633762No Hit
ACTCTGCGTTGATACCACTGCTTAC35670.13574908606271324No Hit
GTATCAACGCAGAGTACATGGGAAG32720.12452229032722115No Hit
GTACATGGGAAGCAGTGGTAAGCAG31520.11995545816363112No Hit
GAGTACATGGTAAGCAGTGGTATCA31060.11820483916758827No Hit
GGTATCAACGCAGAGTACATGGGAA30630.11656839097563519No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30270.11519834132655818No Hit
GCTTACCATGTACTCTGCGTTGATA29270.11139264785689984No Hit
GCAGTGGTATCAACGCAGAGTACAT29200.11112624931402375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29090.11070762303236133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26730.10172618644396764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAC701.0928223E-412.21264613
TCTATAC4150.011.2192053
ACTCCGT855.3166434E-511.1783746
CCGTCAT600.005881697411.08226811
ATCGACC600.005881697411.0822688
GGAACGA2300.010.32430815
GTACTAG1606.1118044E-910.1002761
TCACCGG856.6066295E-410.05709116
ACGATTC951.6473493E-49.99846718
CCTAAAC2002.5465852E-119.9770783
TACTCCG1054.0994622E-59.9540775
TAAGACT3000.09.8187124
GTCCTAA1502.6251837E-79.5061421
CTAGAAC2500.09.501983
CTAGTAC1201.6970665E-59.501983
CGAGACT900.00111193129.501984
TATTCCG2303.6379788E-129.5016175
AACGATT1002.7612367E-49.498363517
CTAGGAC1856.67751E-99.245173
CTATACT3850.09.1317724
Produced by FastQC (version 0.11.5)