FastQCFastQC Report
Wed 25 May 2016
SRR1294578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561071
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC70840.27660303052902474No Hit
GTACATGGTAAGCAGTGGTATCAAC52500.20499236452249858No Hit
ACCATGTACTCTGCGTTGATACCAC51460.20093156339671958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44950.17551251019592976No Hit
GTGGTAAGCAGTGGTATCAACGCAG39270.15333428866282894No Hit
CATGTACTCTGCGTTGATACCACTG35390.13818437677049952No Hit
GTGGTATCAACGCAGAGTACATGGG32200.1257286502404658No Hit
GGTAAGCAGTGGTATCAACGCAGAG29030.11335101603977399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT752.0792964E-411.3973524
GACCGCT700.001495598110.85398611
GTCCTAC1855.456968E-1210.798361
TATACCG909.545905E-510.5526915
TTAGACT2100.010.4023454
CTTAGAC1303.8574035E-710.2281933
TATAACG750.00264509610.1349372
CTTATAC4250.010.0562913
GGGTAAC1606.502705E-89.5128411
GCCTTAG2201.6370905E-119.512841
GTCCTAT1901.027729E-99.512841
TACCGTG800.00452725649.4974225
GTATTAA5750.09.2646791
CTAGGAC1951.6916601E-99.254083
TAGACTC1752.6831913E-89.2260685
TAGACAG3400.09.2180865
TCTAGAC2900.09.1701043
CTAATAC3350.09.0723423
TTATACC3500.08.9550624
TAGAACA3100.08.8850334
Produced by FastQC (version 0.11.5)