FastQCFastQC Report
Wed 25 May 2016
SRR1294578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561071
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75590.29514995874772704No Hit
CCCATGTACTCTGCGTTGATACCAC74560.29112820378661897No Hit
GTACATGGTAAGCAGTGGTATCAAC56030.2187756606513447No Hit
ACCATGTACTCTGCGTTGATACCAC54230.21174735101057332No Hit
GGTATCAACGCAGAGTACTTTTTTT48690.19011577578286584No Hit
GTGGTAAGCAGTGGTATCAACGCAG45970.17949521899236687No Hit
TATCAACGCAGAGTACTTTTTTTTT44140.17234977085758263No Hit
CATGTACTCTGCGTTGATACCACTG36570.14279182420167189No Hit
GGTAAGCAGTGGTATCAACGCAGAG33340.13017991301295434No Hit
GTGGTATCAACGCAGAGTACATGGG32370.126392435039872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT600.00586596311.0862396
TGGCGTA600.005866816311.0860225
TCTATAC3150.010.2566573
GGAGCCG856.5813557E-410.0612645
CTAATAC3500.010.045493
CTAGCAC2105.456968E-129.954993
CGCAGTA800.0045073799.5024912
GTCTTAG2151.05501385E-109.2845861
GTATTAA5900.09.0223081
GTACTAA1804.1804014E-88.9775551
GTATAGG3000.08.8719371
TTAAGCT2151.036824E-98.8395264
ATTAGGC1401.0819393E-58.8237413
TAATACT5450.08.7178814
ACAGGCG2659.094947E-128.6058615
TAGACAG3550.08.5654575
GTACTAG2002.5138434E-88.5550821
GTTCTAT1901.0019721E-78.5050531
GTCATAG2356.0936145E-108.4944081
GTCTAGA2159.616997E-98.4003391
Produced by FastQC (version 0.11.5)