FastQCFastQC Report
Wed 25 May 2016
SRR1294577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055439
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC46600.22671555808759103No Hit
GTACATGGTAAGCAGTGGTATCAAC38350.18657814705277073No Hit
ACCATGTACTCTGCGTTGATACCAC36650.178307407809232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36270.1764586543312645No Hit
GTGGTAAGCAGTGGTATCAACGCAG31100.1513058767494438No Hit
GTGGTATCAACGCAGAGTACATGGG30020.14605152475943095No Hit
CATGTACTCTGCGTTGATACCACTG25350.12333131754335691No Hit
GGTAAGCAGTGGTATCAACGCAGAG21790.10601141653924052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT400.005288489614.2464966
AGTACGG1104.9863957E-711.2245123
TAGGCCC1451.2223609E-911.1354644
TAGGACG700.001492393710.8565872
TGTGCGC803.78292E-410.684092510
CTAGGCC1451.4493708E-810.4801813
CTACACT2850.010.3310444
ACACCGA750.002653599710.1308426
ACTACAC2300.09.9106053
TAGACTG2400.09.8929195
ATTAGAC1252.5785957E-69.877573
CTAGACT1752.582965E-99.7692634
GTTCTAG2151.0913936E-119.7329871
GTGCTAC2251.8189894E-129.7231561
CCGTGCA800.00452475679.4978959
TCTATAC2900.09.4976643
GTCCTAG1651.0455733E-79.2235481
TACACTC3300.09.2094085
GTCTAAA1554.184858E-79.2049521
GTGTTAC2002.5975169E-99.0361941
Produced by FastQC (version 0.11.5)