FastQCFastQC Report
Wed 25 May 2016
SRR1294577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055439
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73360.3569067240623536No Hit
GGTATCAACGCAGAGTACTTTTTTT50090.2436948992405029No Hit
CCCATGTACTCTGCGTTGATACCAC48000.23352675511168175No Hit
TATCAACGCAGAGTACTTTTTTTTT41800.20336288257642282No Hit
GTACATGGTAAGCAGTGGTATCAAC41060.19976267843511775No Hit
ACCATGTACTCTGCGTTGATACCAC39070.19008104837944595No Hit
GTGGTAAGCAGTGGTATCAACGCAG35120.17086374249004715No Hit
GTGGTATCAACGCAGAGTACATGGG32320.1572413484418657No Hit
CATGTACTCTGCGTTGATACCACTG25800.12552063087252893No Hit
GGTAAGCAGTGGTATCAACGCAGAG24600.11968246199473688No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22120.10761691298063333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1950.011.2062763
GCGCTCA600.00587865811.082859
TCGAATA803.7733087E-410.6870349
GTCTAGA1553.6397978E-910.4234661
TACACCG856.588662E-410.05985455
TAACCCG856.5989856E-410.05814110
GTTCTAA1803.9108272E-1010.0317341
GTACTAA1252.5575791E-69.8838981
GTGTAGA1804.154572E-99.5037481
TAGGACT2900.09.5009744
CTTAGGC1002.7529735E-49.5009743
AAGACCG800.00451268169.5009735
GACAGTT1304.240257E-69.4998187
CGAATAG800.0045190269.49935510
TCTATAC2357.2759576E-129.2988243
TATACTG4900.09.1131785
ATAGGAC2201.6734703E-109.0691113
CAGGCGT2106.657501E-109.0485466
CTTACAC3150.09.0485463
TAGGACA2850.09.0009224
Produced by FastQC (version 0.11.5)