FastQCFastQC Report
Wed 25 May 2016
SRR1294576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883136
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC80870.42944322661772705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA63480.3370972675367047No Hit
GTACATGGTAAGCAGTGGTATCAAC56670.3009341863784666No Hit
ACCATGTACTCTGCGTTGATACCAC54630.290101192903752No Hit
GTGGTAAGCAGTGGTATCAACGCAG47140.250327113920609No Hit
CATGTACTCTGCGTTGATACCACTG41220.218890191680261No Hit
GTGGTATCAACGCAGAGTACATGGG35130.1865505199836868No Hit
GGTAAGCAGTGGTATCAACGCAGAG33410.17741681960304514No Hit
GTATCAACGCAGAGTACATGGGAAG24840.1319076264274062No Hit
ACTCTGCGTTGATACCACTGCTTAC24790.1316421118814573No Hit
CCATGTACTCTGCGTTGATACCACT24580.13052695078847198No Hit
GCAGTGGTATCAACGCAGAGTACAT22970.1219773824089179No Hit
GGTATCAACGCAGAGTACATGGGAA21910.11634847403480153No Hit
GCTTACCATGTACTCTGCGTTGATA21880.1161891653072322No Hit
GAGTACATGGTAAGCAGTGGTATCA21410.11369332857531268No Hit
GTACATGGGAAGCAGTGGTATCAAC19360.10280723219140839No Hit
GTACATGGGAAGCAGTGGTAAGCAG19120.10153276237085372No Hit
GCTTACCACTGCTTCCCATGTACTC18920.10047070418705817No Hit
GTACTCTGCGTTGATACCACTGCTT18900.10036449836867863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG701.09351735E-412.2114345
GCGTTAA657.926598E-411.7094811
TAGTACC752.0782482E-411.3976424
GTTCTAG1553.5743142E-910.4346591
GTTCTAT1409.483483E-810.1935211
GCGTGCA856.6117174E-410.0559429
GTCTTAC1502.5921327E-79.5139531
TCCTTAC1304.1600415E-69.5139531
CCTTACA2401.8189894E-129.5013152
CTAGAAC1701.6700142E-89.49803453
CCTAATC800.00452391249.49803453
TAGGACT1606.6555E-89.49803454
TGGACTG2303.6379788E-129.4977825
ACAGCGC1002.763473E-49.4972788
ACCGTCT800.00452688669.4972788
TATGACT1651.06882E-79.2102164
TAAAGGT1356.8647805E-69.14625554
CTAGACT1151.1131243E-49.0850764
TCTTGTA2553.6379788E-128.9424142
GACAGCG850.0074502328.939097
Produced by FastQC (version 0.11.5)