FastQCFastQC Report
Wed 25 May 2016
SRR1294576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883136
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC87660.4655001019575856No Hit
GTATCAACGCAGAGTACTTTTTTTT65980.350372994834149No Hit
GTACATGGTAAGCAGTGGTATCAAC60510.32132570350734097No Hit
ACCATGTACTCTGCGTTGATACCAC58320.30969616639477976No Hit
GTGGTAAGCAGTGGTATCAACGCAG55650.2955176896411093No Hit
GGTATCAACGCAGAGTACTTTTTTT48320.2565932572050027No Hit
CATGTACTCTGCGTTGATACCACTG43100.22887353860793913No Hit
TATCAACGCAGAGTACTTTTTTTTT38900.20657031674823273No Hit
GGTAAGCAGTGGTATCAACGCAGAG37480.1990297036432844No Hit
GTGGTATCAACGCAGAGTACATGGG35290.18740016653072322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27490.14597989736269712No Hit
GTATCAACGCAGAGTACATGGGAAG26330.13981995989668297No Hit
ACTCTGCGTTGATACCACTGCTTAC25900.13753653480152256No Hit
CCATGTACTCTGCGTTGATACCACT25410.1349344922512235No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23910.12696905587275695No Hit
GCAGTGGTATCAACGCAGAGTACAT23810.12643802678085916No Hit
GGTATCAACGCAGAGTACATGGGAA23770.12622561514410005No Hit
GAGTACATGGTAAGCAGTGGTATCA23160.12298633768352366No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22550.11974706022294726No Hit
GCTTACCATGTACTCTGCGTTGATA22200.11788845840130506No Hit
GTACATGGGAAGCAGTGGTAAGCAG21090.1119940354812398No Hit
GTACTCTGCGTTGATACCACTGCTT20540.10907337547580206No Hit
GTACATGGGAAGCAGTGGTATCAAC20340.10801131729200651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC400.00528004514.2498418
GTGCTAG1604.5474735E-1111.2865181
TAAGACT2000.010.9269094
TTATACT2650.010.7565964
ATTATAC2400.010.6893673
TAGACAG1701.382432E-1010.6195035
GATCTAC1002.3867804E-510.4548821
CTGCTCG1201.5240876E-610.2915529
GTCCTAT1303.8172038E-710.2355471
GTCTTAG1606.1245373E-910.0984641
GTCCTAG1803.9108272E-1010.0324611
CGGGTTC951.6475144E-49.9980318
CTAGACT1252.564344E-69.8817264
GTACAAG3850.09.874741
TAACACT1356.4275264E-79.8535734
GTCCAAG2800.09.8438821
GGACTGT2850.09.6686136
GGTTTAT2002.6193447E-109.5044371
GTTCCGG800.00449883579.50443651
CTATCCT1401.0559961E-69.501664
Produced by FastQC (version 0.11.5)