FastQCFastQC Report
Wed 25 May 2016
SRR1294575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623685
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC53110.3270954649454789No Hit
GTACATGGTAAGCAGTGGTATCAAC52990.32635640533724214No Hit
ACCATGTACTCTGCGTTGATACCAC50440.31065138866220976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43350.266985283475551No Hit
GTGGTATCAACGCAGAGTACATGGG37180.2289853019520412No Hit
GTGGTAAGCAGTGGTATCAACGCAG36750.2263370050225259No Hit
CATGTACTCTGCGTTGATACCACTG29140.1794683082001743No Hit
GGTAAGCAGTGGTATCAACGCAGAG27010.16635000015397075No Hit
ATTCCATTCCATTCCATTCCATTCC23440.14436297680892538No Hit
GTATCAACGCAGAGTACTTTTTTTT23160.14263850438970613No Hit
CCATGTACTCTGCGTTGATACCACT22410.138019381838226No Hit
GCTTACCATGTACTCTGCGTTGATA20010.12323818967348962No Hit
GAGTACATGGTAAGCAGTGGTATCA19830.12212960026113441No Hit
GAATGGAATGGAATGGAATGGAATG18980.11689459470279025No Hit
ACTCTGCGTTGATACCACTGCTTAC18860.11615553509455344No Hit
GGTATCAACGCAGAGTACATGGGAA18320.11282976685748775No Hit
GTATCAACGCAGAGTACATGGGAAG17560.10814905600532122No Hit
GGTATCAACGCAGAGTACTTTTTTT16500.10162069613256265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGCG400.00528622614.2471587
TAGACAG2400.012.664535
TACGACC550.003071716812.0892414
TACCGTG550.003073134712.0884977
GGACCGA658.0371957E-411.6899766
GTCCTAT907.337743E-611.62851
GTCTTAG1604.3655746E-1111.2981451
GCTTTAG1104.872909E-711.2440871
GCACCGT600.0058845811.0811226
GTTCTAG1408.1563485E-910.8734031
AGTACTG2050.010.6567395
GAGTACG909.3714654E-510.5713631
GTATAGG1752.2919266E-1010.3297321
GTCTATA1855.820766E-1110.2856511
GGCTAGG856.503176E-410.0738891
ATTAGAC1803.947207E-1010.0257773
TATTCCG951.6467237E-49.9983145
GTATTAT2105.456968E-129.9672851
TTAGACA2107.2759576E-129.9510094
TAGCCTG1252.5748068E-69.8783345
Produced by FastQC (version 0.11.5)