FastQCFastQC Report
Wed 25 May 2016
SRR1294575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623685
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86290.5314454466229596No Hit
GGTATCAACGCAGAGTACTTTTTTT63810.3929949466799287No Hit
GTACATGGTAAGCAGTGGTATCAAC57410.3535784342406317No Hit
CCCATGTACTCTGCGTTGATACCAC55130.33953630168413207No Hit
ACCATGTACTCTGCGTTGATACCAC52450.32303063710017643No Hit
TATCAACGCAGAGTACTTTTTTTTT52210.3215525178837028No Hit
GTGGTAAGCAGTGGTATCAACGCAG41500.2555914478485667No Hit
GTGGTATCAACGCAGAGTACATGGG36370.22399664959644267No Hit
GGTAAGCAGTGGTATCAACGCAGAG31040.1911700853305906No Hit
CATGTACTCTGCGTTGATACCACTG30060.18513443186332323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26580.16370170322445549No Hit
ATTCCATTCCATTCCATTCCATTCC24550.15119927818511594No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24020.14793509824873669No Hit
CCATGTACTCTGCGTTGATACCACT22530.13875844144646282No Hit
GCTTACCATGTACTCTGCGTTGATA21310.13124466876272184No Hit
GAGTACATGGTAAGCAGTGGTATCA21250.13087513895860342No Hit
ACTCTGCGTTGATACCACTGCTTAC19830.12212960026113441No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19320.11898859692612793No Hit
GGTATCAACGCAGAGTACATGGGAA19280.1187422437233823No Hit
GTATCAACGCAGAGTACATGGGAAG18360.11307612006023336No Hit
GAATGGAATGGAATGGAATGGAATG18180.11196753064787812No Hit
GCAGTGGTATCAACGCAGAGTACAT17910.1103046465293453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA17860.10999670502591329No Hit
GTACATGGGAAGCAGTGGTAAGCAG16870.10389946325795951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCT350.0021708916.2850028
GAGCGTC400.005280771314.2493757
GTATTAG1850.011.3041141
TAGACAG2150.011.0490915
GCACCGT700.001488561910.8596786
ATAGGAC1158.789066E-710.7416383
TATTCCG1158.789066E-710.7416385
GCCTTAG1252.2210952E-710.6464211
GTAAGAC1252.2324457E-710.6424853
TTCCGTA909.5189775E-510.5550947
GTCTTAC1451.4293619E-810.4890831
CTAGCAC1409.6521035E-810.1809483
CTAGAAC1909.822543E-1110.0023353
TAGGACA1909.822543E-1110.0023354
TAAGACT1909.822543E-1110.0023354
CTGTCGC1451.6174454E-79.8271559
GCTTAGA800.00449321659.5057331
GTGTAAC1106.790219E-59.5057331
TAATACC2106.548362E-119.5022184
CTTGGAC1901.0459189E-99.5022183
Produced by FastQC (version 0.11.5)