FastQCFastQC Report
Wed 25 May 2016
SRR1294574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179402
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC73220.3359637184879155No Hit
GTACATGGTAAGCAGTGGTATCAAC58690.26929405405703033No Hit
ACCATGTACTCTGCGTTGATACCAC58150.26681631016214535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40370.18523429821574908No Hit
GTGGTAAGCAGTGGTATCAACGCAG39980.18344481651388778No Hit
CATGTACTCTGCGTTGATACCACTG37330.171285517770471No Hit
GGTAAGCAGTGGTATCAACGCAGAG32530.14926112759371607No Hit
GTGGTATCAACGCAGAGTACATGGG31530.1446727129735588No Hit
CCATGTACTCTGCGTTGATACCACT26410.12118003011835357No Hit
GTATCAACGCAGAGTACATGGGAAG24160.1108560972229997No Hit
ACTCTGCGTTGATACCACTGCTTAC22780.10452408504718266No Hit
GAGTACATGGTAAGCAGTGGTATCA22440.1029640240763292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00528851714.246545
AACGTGC752.0796707E-411.3969728
CGCAATC600.005852467411.08954917
ACTGATC2100.010.8542598
AGCACGG803.7804586E-410.6849055
TAAGACT1901.0004442E-109.9980314
TACACGG1356.450118E-79.8512692
CTAATAC2800.09.8373483
CCTAGTA1901.0531949E-99.4994372
CCCTATA1606.643131E-89.4994372
AGCACCG900.00111636739.4976945
GAACCAT1804.2000465E-99.4976946
GCACCGT800.0045257359.4976936
TATACTG4750.09.3977185
GTACTAG2002.6047928E-99.0348341
GTCTAAT1804.145295E-88.9819981
AGGACGG850.0074515868.9390065
ACTGAGC3200.08.9038838
GTCTTAG1502.6625257E-68.87632751
CTAGTAC1401.0879614E-58.8196923
Produced by FastQC (version 0.11.5)