FastQCFastQC Report
Wed 25 May 2016
SRR1294574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179402
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC77050.35353734648311785No Hit
GTATCAACGCAGAGTACTTTTTTTT68970.3164629563522471No Hit
GTACATGGTAAGCAGTGGTATCAAC61450.2819580784086644No Hit
ACCATGTACTCTGCGTTGATACCAC59920.27493780403982376No Hit
GTGGTAAGCAGTGGTATCAACGCAG44090.20230320060273416No Hit
GGTATCAACGCAGAGTACTTTTTTT42980.19721006037435956No Hit
TATCAACGCAGAGTACTTTTTTTTT40410.18541783480055538No Hit
CATGTACTCTGCGTTGATACCACTG38690.17752576165388487No Hit
GGTAAGCAGTGGTATCAACGCAGAG36400.16701829217372471No Hit
GTGGTATCAACGCAGAGTACATGGG31940.14655396296782328No Hit
CCATGTACTCTGCGTTGATACCACT28080.1288426825340162No Hit
GTATCAACGCAGAGTACATGGGAAG25820.11847286549246078No Hit
ACTCTGCGTTGATACCACTGCTTAC23530.10796539601230061No Hit
GAGTACATGGTAAGCAGTGGTATCA22670.10401935943896536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGA550.00307554612.08753717
GTTCTAC1803.6379788E-1211.0901651
GTCACCG750.00265342710.13097215
TAGGACT2450.010.0841424
CCGTGCA951.6449529E-49.9997349
GTAAGGC1252.5626068E-69.8824593
CTAATAC2700.09.8543043
CTATACT3600.09.7663194
GTCTTAG2054.0017767E-119.7377061
GTCCTGG4500.09.7170971
CCCCTAT1201.6884731E-59.5058561
GTCCTAT1201.6884731E-59.5058561
CCTACAC1804.1654857E-99.5023643
GTACTAG1554.223366E-79.1992151
CAGGACT2700.09.1504254
TAGACAG3850.09.1321425
TATACTG4500.09.0800375
GCCTAGT1054.4657028E-49.0531961
GTCTAGA2451.6370905E-118.9238641
CTAGACA2451.6370905E-118.9205874
Produced by FastQC (version 0.11.5)