FastQCFastQC Report
Wed 25 May 2016
SRR1294573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797472
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC34230.19043412080967048No Hit
CCCATGTACTCTGCGTTGATACCAC30290.1685144469566146No Hit
GAATGGAATGGAATGGAATGGAATG27610.15360461804133804No Hit
ACCATGTACTCTGCGTTGATACCAC26830.14926519022271278No Hit
GTACATGGTAAGCAGTGGTATCAAC25450.141587741005145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22770.12667791208986842No Hit
GTGGTATCAACGCAGAGTACATGGG22520.12528706984030905No Hit
GTGGTAAGCAGTGGTATCAACGCAG19190.1067610510761781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT1900.013.4962844
TATTCCG1251.03682396E-1012.9164435
CTAGGAC2000.012.8214693
TCGTGGG1501.8189894E-1212.66353212
CGGTCTC853.95644E-612.2907337
CCTCGGT1052.7303213E-711.7586674
CGCGATC657.986899E-411.69885417
TGCACCG658.043046E-411.6890895
AGCCTCG1900.011.5019542
CTCGTGG1104.9869595E-711.22418111
GCGCGAT600.005874918311.08367416
CTACACG600.00588831911.0802824
CTCGGTC951.3645695E-510.9969725
AGCCGCG909.499675E-510.5573482
GGGCGCG750.002647240210.13364516
CGAATGT750.002649074210.13279915
TAGAACC1502.4549081E-810.1305444
ATAGGAC1606.199116E-910.0909723
GTCTTAC1701.5261321E-910.0711861
CGTGGGC1252.5770605E-69.87782913
Produced by FastQC (version 0.11.5)