FastQCFastQC Report
Wed 25 May 2016
SRR1294573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797472
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50100.2787247868117No Hit
GGTATCAACGCAGAGTACTTTTTTT37290.2074580299442773No Hit
ATTCCATTCCATTCCATTCCATTCC35100.19527425183813713No Hit
CCCATGTACTCTGCGTTGATACCAC31810.17697076783393567No Hit
TATCAACGCAGAGTACTTTTTTTTT31720.17647006462409429No Hit
GTACATGGTAAGCAGTGGTATCAAC27710.15416095494116183No Hit
ACCATGTACTCTGCGTTGATACCAC27700.15410532125117943No Hit
GAATGGAATGGAATGGAATGGAATG27250.15160180520197253No Hit
GTGGTATCAACGCAGAGTACATGGG22820.12695608053978033No Hit
GTGGTAAGCAGTGGTATCAACGCAG21040.11705328372291753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTG855.3307747E-511.17501411
CTAGCAC1106.029084E-610.36603453
GGAACGA1106.0571747E-610.36199815
GTACTAA2053.6379788E-1210.2011721
GGACAGC2350.010.108446
GACAGTC1451.6179911E-79.82712657
GTCCTAC1002.7390078E-49.5056371
GTCTTGC2800.09.5056371
TTAGACT1106.818996E-59.5021984
GTCTAGT1651.0556141E-79.2175871
GTCTAAG1451.6922204E-69.1778571
GAATAGG2600.09.1400361
TAGTACT2002.6429916E-99.0270884
GTATAGA2850.09.0053421
CCCCTAG950.00181392929.0053411
TCTACTC1901.053013E-89.0020833
TAGCACC1701.6783088E-78.9432454
ACAGTCC2252.6193447E-108.8655128
GTCTAGA1951.6312697E-88.7744341
GTAGACC1201.7591694E-48.7103483
Produced by FastQC (version 0.11.5)