FastQCFastQC Report
Wed 25 May 2016
SRR1294572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757503
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC86240.31274671324020314No Hit
ACCATGTACTCTGCGTTGATACCAC63870.23162259478956143No Hit
GTACATGGTAAGCAGTGGTATCAAC58800.2132363953910476No Hit
GTGGTAAGCAGTGGTATCAACGCAG49670.1801267305964853No Hit
CATGTACTCTGCGTTGATACCACTG44500.16137788426703434No Hit
GTGGTATCAACGCAGAGTACATGGG38960.1412872442931159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA38190.13849486292489982No Hit
GGTAAGCAGTGGTATCAACGCAGAG34840.1263461907385051No Hit
GTATCAACGCAGAGTACATGGGAAG28960.10502255119940034No Hit
CCATGTACTCTGCGTTGATACCACT28550.10353569878255799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT1158.859024E-710.7358296
CTAATAC3250.010.5202243
ATTAGAC2053.6379788E-1210.1923593
GGCGTAC750.002655445610.13021856
GTTCTAG2650.09.6917371
CTGGTCG1106.860161E-59.4974249
GGTGCCG1401.0634594E-69.49725210
GTACGTT800.00452879079.497086
TTAGACA2850.09.3308034
CTAAACC2151.07320375E-109.2765544
GTAGGAC1651.07087544E-79.2096233
ACTGATC3250.09.0590828
CAGGACT4100.09.0341374
GCTACAC2002.6702764E-99.0225533
GTCTTAA3700.08.9980851
TTGCGGA850.0073935888.94767919
TTTCGTC850.00739468878.947515517
AGCGAGC850.00745436048.9387538
AGCACCG1502.7144124E-68.8639415
TCCTATA2151.0404619E-98.8380192
Produced by FastQC (version 0.11.5)