FastQCFastQC Report
Wed 25 May 2016
SRR1294572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757503
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC90080.32667235538818995No Hit
GTATCAACGCAGAGTACTTTTTTTT77510.2810876361693895No Hit
ACCATGTACTCTGCGTTGATACCAC66380.24072503275608406No Hit
GTACATGGTAAGCAGTGGTATCAAC59810.2168991293935129No Hit
GTGGTAAGCAGTGGTATCAACGCAG53640.19452381375469038No Hit
GGTATCAACGCAGAGTACTTTTTTT47830.1734540270672416No Hit
TATCAACGCAGAGTACTTTTTTTTT46340.16805058779627802No Hit
CATGTACTCTGCGTTGATACCACTG46300.16790552902390316No Hit
GTGGTATCAACGCAGAGTACATGGG38710.14038062696577303No Hit
GGTAAGCAGTGGTATCAACGCAGAG37920.1375157162113695No Hit
CCATGTACTCTGCGTTGATACCACT31280.11343595999714234No Hit
GTATCAACGCAGAGTACATGGGAAG28740.10422472795133858No Hit
ACTCTGCGTTGATACCACTGCTTAC28280.10255655206902767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC500.001491589513.3099991
GTACCGT700.001489685810.8591956
GTCTACG803.7465594E-410.6955361
AGCACCG1201.5211426E-610.2936125
TCTATAC3950.09.8626243
ATAGGAC2350.09.7039613
GTCTTAA3000.09.5071421
TATACTG4850.09.4038395
ATAGTAC2501.8189894E-129.1217243
GTCCTAA2201.6552804E-109.0751
TTAGGCA1901.05519575E-89.0017014
TAGACTG2850.09.0017015
ACAGCGG950.00182516738.9995788
CCTACAC2651.8189894E-128.9639593
GTATTAG3400.08.9478991
TAGACAG4050.08.9152665
CTACACT3350.08.79270654
TAGGACC1951.6447302E-88.7708884
GTAACCC1304.3609725E-58.7708883
TCTAGAC2502.5465852E-118.74165253
Produced by FastQC (version 0.11.5)