FastQCFastQC Report
Wed 25 May 2016
SRR1294571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814676
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC159030.5650028635622715No Hit
ACCATGTACTCTGCGTTGATACCAC120290.42736712857891995No Hit
GTACATGGTAAGCAGTGGTATCAAC110800.3936509921568237No Hit
GTGGTAAGCAGTGGTATCAACGCAG87330.3102666168326301No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA80590.2863206990786861No Hit
CATGTACTCTGCGTTGATACCACTG79170.28127571343913116No Hit
GGTAAGCAGTGGTATCAACGCAGAG69470.246813487591467No Hit
GTGGTATCAACGCAGAGTACATGGG52740.18737503002121736No Hit
CCATGTACTCTGCGTTGATACCACT51800.18403539164010352No Hit
ACTCTGCGTTGATACCACTGCTTAC51710.18371563902914578No Hit
GTATCAACGCAGAGTACATGGGAAG50540.17955885508669558No Hit
GCAGTGGTATCAACGCAGAGTACAT44150.15685641970869826No Hit
GTACTCTGCGTTGATACCACTGCTT42160.1497863341997445No Hit
GTACATGGGAAGCAGTGGTAAGCAG41140.1461624712755571No Hit
GAGTACATGGTAAGCAGTGGTATCA39590.14065562075350768No Hit
GTACATGATAAGCAGTGGTATCAAC38910.13823971213738276No Hit
GGTATCAACGCAGAGTACATGGGAA38140.13550405091030016No Hit
GCTTACCATGTACTCTGCGTTGATA37320.13259074934379658No Hit
GCGTTGATACCACTGCTTACCATGT36250.12878924608018827No Hit
ATCATGTACTCTGCGTTGATACCAC35870.12743917950058906No Hit
GCTTACCACTGCTTCCCATGTACTC35590.12644439359983173No Hit
GCGTTGATACCACTGCTTACCACTG33330.11841505025800482No Hit
TATCAACGCAGAGTACATGGGAAGC31770.1128726716680712No Hit
CTGCTTACCATGTACTCTGCGTTGA31680.1125529190571135No Hit
GTACATGGGAAGCAGTGGTATCAAC31110.11052781918771469No Hit
AAGCAGTGGTATCAACGCAGAGTAC29790.10583811422700162No Hit
GTATCAACGCAGAGTACATGGTAAG29590.10512755286931781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA601.4691905E-615.83194214
TCTCGTG2050.011.58187710
TTTAGGC1553.1468517E-1011.0299173
GTCTCGT2100.010.8540679
GTAGGAC2100.010.4025543
CGGGTCT1356.4712367E-79.8492366
TCGACCG1356.472819E-79.849069
TCGTGGG3100.09.80454412
CCGCCTA1002.7605327E-49.49865813
CCGAATT1201.7071094E-59.4973099
ATAGAAC2252.7284841E-119.2869173
GTATTAG3000.09.1941251
TAGGACC1356.8712307E-69.1460444
ACCGAAT1356.8743284E-69.1457198
GGTCTAA1252.7175965E-59.1307171
ATCGACC1151.114358E-49.0845438
TACATCG1054.5054755E-49.0453785
TCCCGTA1054.506276E-49.0452177
CTCGTGG2750.08.97959311
GTCTTAC1701.6560443E-78.9516851
Produced by FastQC (version 0.11.5)