FastQCFastQC Report
Wed 25 May 2016
SRR1294571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814676
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC166490.5915068022038771No Hit
ACCATGTACTCTGCGTTGATACCAC124260.4414717715289433No Hit
GTACATGGTAAGCAGTGGTATCAAC112660.400259212783283No Hit
GTGGTAAGCAGTGGTATCAACGCAG102090.36270604502969434No Hit
GTATCAACGCAGAGTACTTTTTTTT94550.3359178818450152No Hit
CATGTACTCTGCGTTGATACCACTG84540.3003542858929411No Hit
GGTAAGCAGTGGTATCAACGCAGAG77350.27480960508420865No Hit
GGTATCAACGCAGAGTACTTTTTTT60770.21590406853222183No Hit
TATCAACGCAGAGTACTTTTTTTTT57210.2032560763654502No Hit
GTGGTATCAACGCAGAGTACATGGG55910.19863742754050556No Hit
CCATGTACTCTGCGTTGATACCACT55770.19814003459012688No Hit
ACTCTGCGTTGATACCACTGCTTAC54980.19533331722727587No Hit
GTATCAACGCAGAGTACATGGGAAG52670.18712633354602803No Hit
GCAGTGGTATCAACGCAGAGTACAT46720.16598713315493505No Hit
GAGTACATGGTAAGCAGTGGTATCA42460.15085217623627017No Hit
GTACATGGGAAGCAGTGGTAAGCAG42290.15024819908223896No Hit
GCTTACCATGTACTCTGCGTTGATA41280.14665986422593577No Hit
GTACTCTGCGTTGATACCACTGCTT41070.14591377480036777No Hit
GGTATCAACGCAGAGTACATGGGAA40130.14257413641925393No Hit
GTACATGATAAGCAGTGGTATCAAC38680.1374225665760464No Hit
GCGTTGATACCACTGCTTACCATGT38180.13564616318183692No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37310.1325552212759124No Hit
GCTTACCACTGCTTCCCATGTACTC36690.13035248106709263No Hit
ATCATGTACTCTGCGTTGATACCAC36510.12971297584517721No Hit
AAGCAGTGGTATCAACGCAGAGTAC35170.12495221474869576No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA34620.12299817101506531No Hit
GCGTTGATACCACTGCTTACCACTG33550.11919666775145701No Hit
TATCAACGCAGAGTACATGGGAAGC33270.1182018818506997No Hit
CTGCTTACCATGTACTCTGCGTTGA32900.11688734333898465No Hit
GTACATGGGAAGCAGTGGTATCAAC31580.11219763837827161No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30630.10882247192927356No Hit
GTATCAACGCAGAGTACATGGTAAG30320.10772110182486368No Hit
ATGGTAAGCAGTGGTATCAACGCAG29190.10370643015395022No Hit
CAGTGGTATCAACGCAGAGTACATG28960.10288928459261386No Hit
GTGATAAGCAGTGGTATCAACGCAG28400.10089971279109923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCG250.006037406618.99774611
TAGCGCG500.001501417413.2984219
ACGGGTC1405.2750693E-1112.2165245
TCGACCG802.8717846E-511.87358959
CACGGGT1451.2150849E-911.1399734
TCTACGC600.00586887411.0855623
AGCGCGC700.001493532710.85585310
TACCCTA951.6418587E-410.0018335
CTAGGAC1856.475602E-109.75871853
GTCTAGA2106.548362E-119.5074861
CCCGGAC1002.7510594E-49.5019113
GGACTGT4000.09.5017416
CCGCGTG1002.7613877E-49.49836519
GTGTATG3200.09.2103771
TAGGACT2401.0913936E-119.1058364
TACTGTC2401.0913936E-119.1038947
CGTGCAC1804.20805E-88.9740273
GCACGGG1804.20805E-88.9740273
GTCTAGT1701.665885E-78.9482221
GTTAGAC850.0074256298.9429753
Produced by FastQC (version 0.11.5)