FastQCFastQC Report
Wed 25 May 2016
SRR1294570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC427561.7739591951214047No Hit
ACCATGTACTCTGCGTTGATACCAC314791.3060731009268103No Hit
GTACATGGTAAGCAGTGGTATCAAC296211.2289841266416675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA290951.2071602297234838No Hit
CATGTACTCTGCGTTGATACCACTG225060.9337806540696589No Hit
GTGGTAAGCAGTGGTATCAACGCAG217480.9023310079404132No Hit
GGTAAGCAGTGGTATCAACGCAGAG188240.7810133756423736No Hit
GTATCAACGCAGAGTACATGGGAAG153970.6388261232875917No Hit
CCATGTACTCTGCGTTGATACCACT152100.6310674375010891No Hit
ACTCTGCGTTGATACCACTGCTTAC145640.6042647047840803No Hit
GGTATCAACGCAGAGTACATGGGAA137820.5718192914950697No Hit
GTACTCTGCGTTGATACCACTGCTT136640.5669234362928917No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA121940.5059326977572833No Hit
GTGGTATCAACGCAGAGTACATGGG119300.49497925899986805No Hit
GTACATGGGAAGCAGTGGTAAGCAG113960.4728234396950961No Hit
GCAGTGGTATCAACGCAGAGTACAT111200.4613721173577982No Hit
GAGTACATGGTAAGCAGTGGTATCA107670.4467260420495876No Hit
GCTTACCATGTACTCTGCGTTGATA105020.4357311129938486No Hit
TATCAACGCAGAGTACATGGGAAGC99790.41403168697063564No Hit
GCGTTGATACCACTGCTTACCATGT96830.40175055866686693No Hit
GCTTACCACTGCTTCCCATGTACTC95660.3968961937630124No Hit
GCGTTGATACCACTGCTTACCACTG95160.3948216788468352No Hit
GTACATGGGAAGCAGTGGTATCAAC92580.38411718187936117No Hit
AAGCAGTGGTATCAACGCAGAGTAC87140.36154645959135373No Hit
CTGCTTACCATGTACTCTGCGTTGA86930.36067516332655936No Hit
GTATCAACGCAGAGTACATGGTAAG78590.3260722545247245No Hit
GTACATGATAAGCAGTGGTATCAAC71610.2971120262948915No Hit
CAGTGGTATCAACGCAGAGTACATG70190.29122040393294835No Hit
ATCATGTACTCTGCGTTGATACCAC69900.2900171852815656No Hit
TATCAACGCAGAGTACATGGTAAGC69220.2871958449955647No Hit
ACGCAGAGTACATGGTAAGCAGTGG68210.28300532486488683No Hit
ATGGTAAGCAGTGGTATCAACGCAG67970.2820095577051218No Hit
ACGCAGAGTACATGGGAAGCAGTGG62280.25840157795902585No Hit
GGTATCAACGCAGAGTACATGGTAA57580.23890113774696062No Hit
GTACATAAGCAGTGGTATCAACGCA57530.2386936862553429No Hit
GTATCAACGCAGAGTACTTTTTTTT53980.22396463035048517No Hit
GTGGTATCAACGCAGAGTACATGGT50150.20807384609256818No Hit
TACCACTGCTTACCATGTACTCTGC48930.20301202969709595No Hit
GAGTACATGGGAAGCAGTGGTAAGC48720.20214073343230152No Hit
GTGATAAGCAGTGGTATCAACGCAG46500.192929887204475No Hit
AAAAAGTACTCTGCGTTGATACCAC44670.1853371626112666No Hit
GAGTACATGGGAAGCAGTGGTATCA40790.16923892686173192No Hit
AAAGTACTCTGCGTTGATACCACTG37990.15762164333113987No Hit
GCTTCCCATGTACTCTGCGTTGATA37620.15608650229316878No Hit
TATCAACGCAGAGTACTTTTTTTTT36860.15293323962057953No Hit
GGTATCAACGCAGAGTACTTTTTTT36730.15239386574237346No Hit
GTACATGGTAAGCAGTGGTAAGCAG36400.15102468589769652No Hit
GTAAGCAGTGGTATCAACGCAGAGT36050.14957252545637253No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT35950.1491576224731371No Hit
GCAGAGTACATGGTAAGCAGTGGTA34950.1450085926407828No Hit
TGGTAAGCAGTGGTATCAACGCAGA34700.14397133518269423No Hit
ACTCTGCGTTGATACCACTGCTTAT34200.14189682026651707No Hit
CTTCCCATGTACTCTGCGTTGATAC33390.1385361061023101No Hit
GCTTATGTACTCTGCGTTGATACCA33240.13791375162745695No Hit
GATAAGCAGTGGTATCAACGCAGAG33080.13724990685428026No Hit
CTGCTTACCACTGCTTCCCATGTAC31570.13098487180742527No Hit
GAGTAAGCAGTGGTATCAACGCAGA30980.1285369442063362No Hit
GCGTTGATACCACTGCTTCCCATGT30480.12646242929015908No Hit
GCTTACCACTGCTTACCATGTACTC30390.12608901660524718No Hit
GATACCACTGCTTACCATGTACTCT29790.12359959870583462No Hit
GTATCAACGCAGAGTACATGGGAAA29270.12144210319301038No Hit
GTTGATACCACTGCTTACCATGTAC28440.1179984084321563No Hit
GAGTACATGATAAGCAGTGGTATCA27820.11542600993609664No Hit
GAACAAAAAAAAAAAAAAAAAAAAA27610.11455471367130224No Hit
ATACCACTGCTTACCATGTACTCTG26410.10957587787247706No Hit
CTGCTTCCCATGTACTCTGCGTTGA26200.10870458160768268No Hit
GCTTATCATGTACTCTGCGTTGATA25930.107584343552947No Hit
GTACATGGGAAAGCAGTGGTAAGCA25340.10513641595185796No Hit
GTACATGGGAAGTAAGCAGTGGTAT25300.10497045475856381No Hit
GTTGATACCACTGCTTACCACTGCT24500.10165123089268037No Hit
CCACTGCTTACCATGTACTCTGCGT24270.10069695403123888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG751.458167E-512.6844741
GTACTAA751.458167E-512.6844741
GATCAGT604.102353E-412.6634156
GTGCTAG1251.380613E-912.1770951
GGTCTAA550.003037162812.1079071
CCTAAGC1552.5465852E-1111.6428973
GAACAAA7850.011.6341661
GCAAAAA9700.011.57294751
CCTAATA1001.9324634E-611.3992032
TCTAGGG752.0760651E-411.3992032
CTAGATT803.7805305E-410.68497854
AGTACTA750.002654120810.1307335
TAGCTAA951.6489415E-49.9974335
AGGGTAA1151.030028E-59.9104995
GTTCTAG1356.323444E-79.8657021
GGACCAT1356.468799E-79.8493236
TATACTG1451.6240665E-79.8250645
CCCAAAA5350.09.6022651
GTATTAA2002.582965E-109.5133561
GTATAGA1401.038954E-69.5133561
Produced by FastQC (version 0.11.5)