FastQCFastQC Report
Wed 25 May 2016
SRR1294570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410202
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC455191.888596889389354No Hit
ACCATGTACTCTGCGTTGATACCAC329641.3676861939372718No Hit
GTACATGGTAAGCAGTGGTATCAAC308011.2779426786634482No Hit
GTGGTAAGCAGTGGTATCAACGCAG247281.0259720969445714No Hit
CATGTACTCTGCGTTGATACCACTG235140.9756028747797901No Hit
GGTAAGCAGTGGTATCAACGCAGAG214180.8886392094936441No Hit
GTATCAACGCAGAGTACTTTTTTTT188280.7811793368356676No Hit
GTATCAACGCAGAGTACATGGGAAG164660.6831792521954592No Hit
CCATGTACTCTGCGTTGATACCACT164060.6806898342960466No Hit
ACTCTGCGTTGATACCACTGCTTAC153740.6378718464261501No Hit
GGTATCAACGCAGAGTACATGGGAA144730.6004890876366379No Hit
GTACTCTGCGTTGATACCACTGCTT133580.5542274050058874No Hit
TATCAACGCAGAGTACTTTTTTTTT126730.5258065506542605No Hit
GTACTTTTTTTTTTTTTTTTTTTTT125480.5206202633638177No Hit
GTGGTATCAACGCAGAGTACATGGG123610.5128615775773151No Hit
GGTATCAACGCAGAGTACTTTTTTT122820.5095838440097552No Hit
GCAGTGGTATCAACGCAGAGTACAT121540.5042730858243417No Hit
GTACATGGGAAGCAGTGGTAAGCAG119830.49717824481101586No Hit
GAGTACATGGTAAGCAGTGGTATCA116240.48228322771286386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA111710.46348812257229893No Hit
GCTTACCATGTACTCTGCGTTGATA109810.4556049658908257No Hit
TATCAACGCAGAGTACATGGGAAGC108260.44917396965067663No Hit
AAGCAGTGGTATCAACGCAGAGTAC106020.4398801428262029No Hit
GCGTTGATACCACTGCTTACCATGT100700.4178073041180781No Hit
GCGTTGATACCACTGCTTACCACTG98690.40946775415504594No Hit
GCTTACCACTGCTTCCCATGTACTC96770.4015016168769257No Hit
GTACATGGGAAGCAGTGGTATCAAC96080.3986387862926012No Hit
CTGCTTACCATGTACTCTGCGTTGA90930.37727128265597654No Hit
GTATCAACGCAGAGTACATGGTAAG83710.3473152872663785No Hit
CAGTGGTATCAACGCAGAGTACATG78920.32744143436940143No Hit
ATGGTAAGCAGTGGTATCAACGCAG78030.32374879781860605No Hit
GTACATGATAAGCAGTGGTATCAAC74910.3108038247416607No Hit
ATCATGTACTCTGCGTTGATACCAC73660.3056175374512178No Hit
ACGCAGAGTACATGGTAAGCAGTGG73440.30470475088809984No Hit
TATCAACGCAGAGTACATGGTAAGC72530.30092913374065744No Hit
GAGTACTTTTTTTTTTTTTTTTTTT71980.29864716733286256No Hit
ACGCAGAGTACATGGGAAGCAGTGG70860.29400025392062573No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65960.27367000774208966No Hit
GGTATCAACGCAGAGTACATGGTAA63450.2632559428628804No Hit
GTACATAAGCAGTGGTATCAACGCA56180.23309249598166462No Hit
GTGATAAGCAGTGGTATCAACGCAG52300.21699426023212995No Hit
GAGTACATGGGAAGCAGTGGTAAGC52250.2167868087405122No Hit
GTGGTATCAACGCAGAGTACATGGT51920.21541762889583527No Hit
TACCACTGCTTACCATGTACTCTGC50570.209816438622157No Hit
GTGGTATCAACGCAGAGTACTTTTT46470.19280541630950435No Hit
AAAAAGTACTCTGCGTTGATACCAC46260.19193412004470994No Hit
GAGTACATGGGAAGCAGTGGTATCA44380.18413394395988386No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT40920.16977830073993797No Hit
GTAAGCAGTGGTATCAACGCAGAGT40700.16886551417682003No Hit
TGGTAAGCAGTGGTATCAACGCAGA39480.1638036977813478No Hit
GTACATGGTAAGCAGTGGTAAGCAG39330.16318134330649464No Hit
AAAGTACTCTGCGTTGATACCACTG39080.16214408584840606No Hit
GCTTCCCATGTACTCTGCGTTGATA38460.1595716873523464No Hit
GCAGAGTACATGGTAAGCAGTGGTA38260.15874188138587556No Hit
GATAAGCAGTGGTATCAACGCAGAG38070.15795356571772823No Hit
ACTCTGCGTTGATACCACTGCTTAT35250.1462533015904891No Hit
GCTTATGTACTCTGCGTTGATACCA34310.14235321354807604No Hit
GAGTAAGCAGTGGTATCAACGCAGA33900.1406521113168108No Hit
CTTCCCATGTACTCTGCGTTGATAC33720.139905285946987No Hit
CTGCTTACCACTGCTTCCCATGTAC33250.1379552419257805No Hit
GTATCAACGCAGAGTACATGGGAAA32500.13484346955151477No Hit
GATACCACTGCTTACCATGTACTCT31300.1298646337526896No Hit
GCGTTGATACCACTGCTTCCCATGT30890.12816353152142435No Hit
GCTTACCACTGCTTACCATGTACTC30000.12447089497062902No Hit
GAGTACATGATAAGCAGTGGTATCA29120.12081974871815723No Hit
GTTGATACCACTGCTTACCATGTAC28510.11828884052042112No Hit
GCAGAGTACTTTTTTTTTTTTTTTT28200.11700264127239128No Hit
GCAGTGGTATCAACGCAGAGTACTT27630.11463769426794933No Hit
CTGCTTCCCATGTACTCTGCGTTGA26690.11073760622553627No Hit
ATACCACTGCTTACCATGTACTCTG26630.11048866443559503No Hit
GTACATGGGAAGTAAGCAGTGGTAT26250.10891203309930039No Hit
GTACATGGGAAAGCAGTGGTAAGCA26180.1086216010110356No Hit
GCAGTGGTAAGCAGTGGTATCAACG26140.10845563981774142No Hit
CCACTGCTTACCATGTACTCTGCGT25840.10721093086803514No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT25530.1059247316200053No Hit
GCTTATCATGTACTCTGCGTTGATA25500.10580026072503468No Hit
CATGGTAAGCAGTGGTATCAACGCA25300.10497045475856381No Hit
GTTGATACCACTGCTTACCACTGCT25020.1038087264055046No Hit
ATCAACGCAGAGTACATGGGAAGCA24960.10355978461556335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT500.00150136113.29835810
AAGACCG701.08904715E-412.2170885
CCCTAAC550.003054031612.0989571
TGTACCG550.003064020312.0936835
GTGTCGA550.003073081812.08891513
ATTCCGT658.0080144E-411.6954776
TCTTAGG1053.456953E-610.8600852
TCGGACT700.001489066910.8596333
AAAAGTA10950.010.8476872
GCTTAGG1502.1118467E-910.7738331
TAAGACT1106.031987E-610.36601454
GTACTAC1409.600808E-810.1853461
AGGTCGT750.002642530210.1360796
GTCTAGG856.555525E-410.0655191
TGCAATA856.5793423E-410.0615492
TAGGACT1803.929017E-1010.0300784
CCCTAGA951.6336693E-410.0066571
TCCTAGC951.6401854E-410.0027092
TAAGGCT951.6408725E-410.0022954
AAAAAGT11700.09.9938271
Produced by FastQC (version 0.11.5)