FastQCFastQC Report
Wed 25 May 2016
SRR1294569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591396
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC45580.28641519772577034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41240.2591435444100651No Hit
GTACATGGTAAGCAGTGGTATCAAC37870.23796716844833088No Hit
ACCATGTACTCTGCGTTGATACCAC36660.23036378123358361No Hit
ATTCCATTCCATTCCATTCCATTCC36170.22728472360116525No Hit
GAATGGAATGGAATGGAATGGAATG30190.1897076529034885No Hit
GTGGTAAGCAGTGGTATCAACGCAG29040.1824812931539353No Hit
GTGGTATCAACGCAGAGTACATGGG28400.17845966685853176No Hit
CATGTACTCTGCGTTGATACCACTG23110.14521841201058694No Hit
GGTAAGCAGTGGTATCAACGCAGAG19550.12284811574240478No Hit
GTATCAACGCAGAGTACTTTTTTTT16490.10361971501750664No Hit
CCATGTACTCTGCGTTGATACCACT16310.1024886326219244No Hit
ACTCTGCGTTGATACCACTGCTTAC16200.10179741560240191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAA604.0984649E-412.6643412
ACACCGT604.100639E-412.6635456
GAACGAT700.001492939410.85583116
GACAGCC1252.247125E-710.6373787
CTAGTAC1355.7034413E-810.5532863
TAGGACC856.606173E-410.0566614
CCAGGAC2107.2759576E-129.950243
CTAGGAC1151.0288139E-59.9109123
GTTTAGA1451.5795558E-79.8431061
GTCTAAA1701.6210834E-89.5150031
TATGGAC800.00452356469.4979573
GTAGAAC1304.2486117E-69.4979573
TCTAGAC1201.70425E-59.4979563
TATTCCG900.00111605689.4976595
TAGACAG2252.7284841E-119.2865995
TGGACAG1951.6880222E-99.2541295
GTCCTAG1651.0393342E-79.226671
CCTACAC1651.0683834E-79.210143
TTATACT2600.09.1326514
GAATTAG950.00179750769.0142141
Produced by FastQC (version 0.11.5)