FastQCFastQC Report
Wed 25 May 2016
SRR1294569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591396
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64910.40788087942913015No Hit
GGTATCAACGCAGAGTACTTTTTTT47830.30055372767054833No Hit
CCCATGTACTCTGCGTTGATACCAC47800.30036521393795135No Hit
GTACATGGTAAGCAGTGGTATCAAC40280.2531111049669598No Hit
TATCAACGCAGAGTACTTTTTTTTT39250.24663880014779474No Hit
ACCATGTACTCTGCGTTGATACCAC38480.2418002810111374No Hit
ATTCCATTCCATTCCATTCCATTCC38000.23878406128958474No Hit
GTGGTAAGCAGTGGTATCAACGCAG32910.20679956465895355No Hit
GAATGGAATGGAATGGAATGGAATG30430.19121576276426483No Hit
GTGGTATCAACGCAGAGTACATGGG29930.18807386722098082No Hit
CATGTACTCTGCGTTGATACCACTG24690.1551468019273644No Hit
GGTAAGCAGTGGTATCAACGCAGAG23840.1498055795037816No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20510.1288805551855101No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19630.12335081902933023No Hit
CCATGTACTCTGCGTTGATACCACT19030.11958054437738941No Hit
GTATCAACGCAGAGTACATGGGAAG17400.10933796490628354No Hit
ACTCTGCGTTGATACCACTGCTTAC16500.10368255292837233No Hit
GGTATCAACGCAGAGTACATGGGAA16340.10267714635452145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACC701.0920441E-412.2129548
TATGGGT855.3117023E-511.1787634
TGCTAGC700.001488495610.8597122
GGACCCT909.494717E-510.5577216
TAGGACT1303.8293365E-710.2328684
CCGTGTG1409.699943E-810.1774619
TAAGGCC750.00264312910.1354124
GAACGAA1151.0279044E-59.91165116
CTGGACC1252.5624176E-69.8820264
CTAGGAC1106.819631E-59.5019483
ACTGCGT900.00111465639.4989638
GTATTAG2151.05501385E-109.2856431
GTATAGG1752.636807E-89.2351091
GCCTTAG1451.6890663E-69.1789111
TTAAGGT1252.7464683E-59.121874
GGAACGA1252.7576836E-59.11871815
TATTCCG1151.1078527E-49.088825
GGCTTAG1054.459918E-49.05402851
TCTCGCT950.00181958739.002132
TTAGGGC850.00742242678.943014
Produced by FastQC (version 0.11.5)