FastQCFastQC Report
Wed 25 May 2016
SRR1294568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434605
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAAC98490.40454200989482897No Hit
CCCATGTACTCTGCGTTGATACCAC95720.39316439422411437No Hit
ACCATGTACTCTGCGTTGATACCAC93160.3826493414742843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA62330.2560168898034794No Hit
GTGGTAAGCAGTGGTATCAACGCAG54330.22315734996026051No Hit
CATGTACTCTGCGTTGATACCACTG53360.2191731307542702No Hit
GGTAAGCAGTGGTATCAACGCAGAG48500.1992109602995147No Hit
GTGGTATCAACGCAGAGTACATGGG45760.18795656790321225No Hit
CCATGTACTCTGCGTTGATACCACT41200.16922663019257744No Hit
GAGTACATGGTAAGCAGTGGTATCA37170.15267363699655592No Hit
ATTCCATTCCATTCCATTCCATTCC35710.14667677097516846No Hit
ACTCTGCGTTGATACCACTGCTTAC33830.138954779112012No Hit
GTATCAACGCAGAGTACATGGGAAG32380.13299898751542857No Hit
GCTTACCATGTACTCTGCGTTGATA32140.132013201320132No Hit
GCGTTGATACCACTGCTTACCATGT30850.12671460052041297No Hit
GCAGTGGTATCAACGCAGAGTACAT28920.11878723653323639No Hit
GAATGGAATGGAATGGAATGGAATG28440.11681566414264326No Hit
GGTATCAACGCAGAGTACATGGGAA27910.11463871962803002No Hit
GTACTCTGCGTTGATACCACTGCTT27180.1116402866173363No Hit
GTACATGGGAAGCAGTGGTAAGCAG24640.10120738271711428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAT602.5753663E-514.24651811
ACGGGTC500.001502504613.2970245
GATCGCC550.003074926612.087954511
TTTCGTC657.990246E-411.69858317
CGAATTA658.034446E-411.69089215
TACACCG600.00588707711.0808535
GTACTAA1553.074092E-1011.0445361
TACATCG803.780136E-410.6851085
TTACACC1803.6379788E-1110.553414
GTTCTAG2350.010.5223351
GCCCCGA1002.406384E-510.44744811
CTTAGAC1303.8534017E-710.22893
CTGTCGC1409.726682E-810.1762939
ACCGACT750.002653413710.1310658
CGTACAC900.00111585389.4982643
CTAGGAC1304.250289E-69.4982643
AAAACCG900.00111627319.4978735
TGTCGCC1554.2773172E-79.19130210
GTGTTAA2700.09.1583281
CTAATAC2401.0913936E-119.1025033
Produced by FastQC (version 0.11.5)