FastQCFastQC Report
Wed 25 May 2016
SRR1294568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434605
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAAC101220.4157553278663274No Hit
CCCATGTACTCTGCGTTGATACCAC100650.4134140856524981No Hit
ACCATGTACTCTGCGTTGATACCAC94350.38753719802596315No Hit
GTATCAACGCAGAGTACTTTTTTTT93270.3831011601471286No Hit
GGTATCAACGCAGAGTACTTTTTTT65420.26870888706792273No Hit
GTGGTAAGCAGTGGTATCAACGCAG59210.24320166926462403No Hit
TATCAACGCAGAGTACTTTTTTTTT57200.23494570987901528No Hit
CATGTACTCTGCGTTGATACCACTG56640.23264554208998994No Hit
GGTAAGCAGTGGTATCAACGCAGAG52430.215353209247496No Hit
GTGGTATCAACGCAGAGTACATGGG46950.19284442445489106No Hit
CCATGTACTCTGCGTTGATACCACT43810.1799470550664276No Hit
GAGTACATGGTAAGCAGTGGTATCA39430.16195645700226524No Hit
ACTCTGCGTTGATACCACTGCTTAC36050.14807330141850525No Hit
ATTCCATTCCATTCCATTCCATTCC35470.14569098477987188No Hit
GTATCAACGCAGAGTACATGGGAAG33960.13948874663446434No Hit
GCTTACCATGTACTCTGCGTTGATA33760.13866725813838385No Hit
GCAGTGGTATCAACGCAGAGTACAT32590.13386155043631306No Hit
GCGTTGATACCACTGCTTACCATGT31240.12831650308776987No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29320.12043021352539733No Hit
GGTATCAACGCAGAGTACATGGGAA29050.1193212040556887No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28970.11899260865725651No Hit
GTACTCTGCGTTGATACCACTGCTT28080.1153369848496984No Hit
GAATGGAATGGAATGGAATGGAATG27910.11463871962803002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26220.10769714183615No Hit
GTACATGGGAAGCAGTGGTAAGCAG25970.1066702812160494No Hit
GTATCAACGCAGAGTACATGGTAAG24690.10141275484113439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG400.005275886514.2517725
ATAACGC550.003066446912.0924133
TGTACCG1001.9279814E-611.4014185
TTTCGTC803.776589E-410.68619417
TACCGTG1002.3977012E-510.450877
CTAGACA2053.6379788E-1210.196394
ATTTTCG750.002650274210.13251215
GTGTAGG2650.09.6850241
GTATTGG2600.09.5056731
GTATAGA2700.09.5056731
GTCCAAT1106.7944406E-59.5056731
TACGCAG800.004512379.5011825
TTTTCGT800.00452155439.49883916
CTAATAC2650.09.3219143
GTCTTAG1951.6643753E-99.2619371
TCTAGAC1651.0640906E-79.2132663
AGCACCG1151.1094714E-49.0880875
CGAATTA950.00182557688.9992715
CTAGACT1606.731134E-78.9073574
CCTAGAC1502.698056E-68.8677693
Produced by FastQC (version 0.11.5)