FastQCFastQC Report
Wed 25 May 2016
SRR1294567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926960
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC252620.8630797824363845No Hit
ACCATGTACTCTGCGTTGATACCAC206610.7058859704266542No Hit
GTACATGGTAAGCAGTGGTATCAAC202790.6928348867083937No Hit
GTGGTAAGCAGTGGTATCAACGCAG150020.5125454396370296No Hit
CATGTACTCTGCGTTGATACCACTG136060.46485090332631807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA135340.4623910132014103No Hit
GGTAAGCAGTGGTATCAACGCAGAG118000.4031486593598819No Hit
CCATGTACTCTGCGTTGATACCACT90780.31015114658211934No Hit
GTATCAACGCAGAGTACATGGGAAG86660.2960751086451472No Hit
ACTCTGCGTTGATACCACTGCTTAC85510.2921461174734195No Hit
GTGGTATCAACGCAGAGTACATGGG78470.2680938584743215No Hit
GTACATGGGAAGCAGTGGTAAGCAG78470.2680938584743215No Hit
GAGTACATGGTAAGCAGTGGTATCA74050.25299286631863777No Hit
GCAGTGGTATCAACGCAGAGTACAT72900.2490638751469101No Hit
GCTTACCATGTACTCTGCGTTGATA71100.2429141498346407No Hit
GTACTCTGCGTTGATACCACTGCTT70230.23994178260037718No Hit
GCTTACCACTGCTTCCCATGTACTC67410.23030721294448847No Hit
GTACATGATAAGCAGTGGTATCAAC66510.22723235028835378No Hit
GCGTTGATACCACTGCTTACCATGT65820.2248749555853172No Hit
GGTATCAACGCAGAGTACATGGGAA64200.21934020280427474No Hit
ATCATGTACTCTGCGTTGATACCAC62140.21230218383578867No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA56230.19211058572717085No Hit
GCGTTGATACCACTGCTTACCACTG55710.19033399841473747No Hit
CTGCTTACCATGTACTCTGCGTTGA53650.18329597944625142No Hit
GTATCAACGCAGAGTACATGGTAAG52350.17885451116516796No Hit
TATCAACGCAGAGTACATGGGAAGC52190.17830786891518846No Hit
GTACATGGGAAGCAGTGGTATCAAC49940.17062071227485173No Hit
AAGCAGTGGTATCAACGCAGAGTAC47850.1634801978844945No Hit
GTACATAAGCAGTGGTATCAACGCA46630.1593120507284008No Hit
CAGTGGTATCAACGCAGAGTACATG45010.15377729794735837No Hit
ATGGTAAGCAGTGGTATCAACGCAG42910.14660261841637742No Hit
ACGCAGAGTACATGGTAAGCAGTGG40720.13912045261978298No Hit
TATCAACGCAGAGTACATGGTAAGC40520.13843714980730862No Hit
GTGATAAGCAGTGGTATCAACGCAG39060.13344903927624566No Hit
GTATCAACGCAGAGTACTTTTTTTT34870.11913384535490747No Hit
ACGCAGAGTACATGGGAAGCAGTGG33420.11417989996446827No Hit
GGTATCAACGCAGAGTACATGGTAA32380.11062672533960151No Hit
GAGTACATGGGAAGCAGTGGTAAGC31830.10874764260529696No Hit
TACCACTGCTTACCATGTACTCTGC30440.10399868805860005No Hit
GTGGTATCAACGCAGAGTACATGGT30330.10362287151173914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA752.0794506E-411.3973754
TGGATCG600.00588409911.08172814
ATACCGT600.00588709311.0809726
CTAGGAC1900.010.9974673
GTATTAG2700.010.9220651
TAGACTG2100.010.854835
GTATAGG2650.010.769171
ATAGACG803.780854E-410.68503953
GTTCTAA2100.010.4187451
TGGATAG1553.6798156E-910.4171345
GTCCTAG1303.774676E-710.2445181
GTCTTAG1752.5174813E-99.7845591
GTCCTAA1502.599245E-79.5127661
GTATCGA800.0044676919.5127661
AGCTCGT1002.7626133E-49.49797511
GTGCTAG2054.129106E-109.2807471
GTCTATA1951.6443664E-99.2688491
CTAGACT1651.07031155E-79.214
GTGTAAG4150.09.1689321
GTATAGA3450.09.0991681
Produced by FastQC (version 0.11.5)