FastQCFastQC Report
Wed 25 May 2016
SRR1294567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926960
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC266120.9092027222784049No Hit
ACCATGTACTCTGCGTTGATACCAC217100.7417252029409354No Hit
GTACATGGTAAGCAGTGGTATCAAC207850.7101224478639954No Hit
GTGGTAAGCAGTGGTATCAACGCAG167060.5707628392598464No Hit
CATGTACTCTGCGTTGATACCACTG141560.4836417306693634No Hit
GTATCAACGCAGAGTACTTTTTTTT139840.47776532648208375No Hit
GGTAAGCAGTGGTATCAACGCAGAG128320.43840708448355975No Hit
CCATGTACTCTGCGTTGATACCACT97790.33410091015934623No Hit
GGTATCAACGCAGAGTACTTTTTTT93180.31835078033181186No Hit
GTATCAACGCAGAGTACATGGGAAG90760.31008281630087187No Hit
ACTCTGCGTTGATACCACTGCTTAC85940.2936152185202394No Hit
TATCAACGCAGAGTACTTTTTTTTT84860.2899253833328778No Hit
GTGGTATCAACGCAGAGTACATGGG81950.2799833274113756No Hit
GTACATGGGAAGCAGTGGTAAGCAG80990.27670347391149863No Hit
GCAGTGGTATCAACGCAGAGTACAT80800.2760543362396479No Hit
GAGTACATGGTAAGCAGTGGTATCA78140.2669664088337388No Hit
GCTTACCATGTACTCTGCGTTGATA74140.25330035258425126No Hit
GTACTCTGCGTTGATACCACTGCTT71320.24366578292836252No Hit
GCGTTGATACCACTGCTTACCATGT70670.2414450487878208No Hit
GTACATGATAAGCAGTGGTATCAAC68840.23519282805368028No Hit
GCTTACCACTGCTTCCCATGTACTC68830.23515866291305654No Hit
GGTATCAACGCAGAGTACATGGGAA66170.22607073550714735No Hit
ATCATGTACTCTGCGTTGATACCAC63910.21834941372618688No Hit
AAGCAGTGGTATCAACGCAGAGTAC57400.19610790718014595No Hit
GCGTTGATACCACTGCTTACCACTG57230.19552709978954272No Hit
TATCAACGCAGAGTACATGGGAAGC57160.1952879438051767No Hit
CTGCTTACCATGTACTCTGCGTTGA57030.19484379697706836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA55980.19125645721157788No Hit
GTATCAACGCAGAGTACATGGTAAG54400.18585836499303032No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51920.17738541011834805No Hit
GTACATGGGAAGCAGTGGTATCAAC50680.17314893268100692No Hit
CAGTGGTATCAACGCAGAGTACATG47910.16368518872823679No Hit
ATGGTAAGCAGTGGTATCAACGCAG47070.16081531691584441No Hit
GTACATAAGCAGTGGTATCAACGCA46470.1587654084784213No Hit
ACGCAGAGTACATGGTAAGCAGTGG44780.15299149971301282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43920.150053297619373No Hit
TATCAACGCAGAGTACATGGTAAGC43780.14957498565064095No Hit
GTGATAAGCAGTGGTATCAACGCAG42430.1449626916664389No Hit
ACGCAGAGTACATGGGAAGCAGTGG38240.13064749774510073No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37970.12972503894826032No Hit
GAGTACATGGGAAGCAGTGGTAAGC36030.12309700166725887No Hit
GGTATCAACGCAGAGTACATGGTAA34500.11786973515182989No Hit
TACCACTGCTTACCATGTACTCTGC31540.10775685352720911No Hit
GTGGTATCAACGCAGAGTACATGGT31490.10758602782409052No Hit
GCTTATGTACTCTGCGTTGATACCA29470.10068466941809934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC600.00588389911.0817798
GTCTTAC1752.0008883E-1110.8639721
TCTATAC1855.456968E-1210.7868793
CGTTAAT856.6072366E-410.05706914
CTAGGAC1803.929017E-1010.0306553
TCTGTCG1054.1139116E-59.9509858
CCGTGCA1252.5765112E-69.8786149
GTCTAGG1554.028152E-89.812621
GTCTAGA1554.028152E-89.812621
TAGACTG1856.4574124E-109.7595575
TTAGACT2054.1836756E-119.73454
CCCCTAT1106.793342E-59.5059751
ACACCGT1201.6953558E-59.5028896
TAGACAG3300.09.5027265
TAGGACC1304.226031E-69.5027264
AACCGAT800.00451886849.4996417
CGTGCAT800.0045226919.49866810
ACCGTGC1451.7107177E-69.1711278
GTATAAT3950.09.1449891
GTATTAG2106.6029315E-109.053311
Produced by FastQC (version 0.11.5)