FastQCFastQC Report
Wed 25 May 2016
SRR1294566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610591
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAAC116530.44637402028889245No Hit
CCCATGTACTCTGCGTTGATACCAC112010.42905993317222035No Hit
ACCATGTACTCTGCGTTGATACCAC101000.3868855749521852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA72880.2791705020051015No Hit
GTGGTAAGCAGTGGTATCAACGCAG72010.2758379232901669No Hit
GGTAAGCAGTGGTATCAACGCAGAG58240.22309124638826994No Hit
CATGTACTCTGCGTTGATACCACTG57490.2202183337029814No Hit
CCATGTACTCTGCGTTGATACCACT46180.17689481040883082No Hit
GTGGTATCAACGCAGAGTACATGGG43890.16812285034308322No Hit
GAGTACATGGTAAGCAGTGGTATCA41260.1580485031933382No Hit
ACTCTGCGTTGATACCACTGCTTAC39240.1503107916942945No Hit
GCTTACCATGTACTCTGCGTTGATA37920.14525446536818673No Hit
GCGTTGATACCACTGCTTACCATGT36240.1388191409531405No Hit
GTATCAACGCAGAGTACATGGGAAG35640.1365208108049097No Hit
GCAGTGGTATCAACGCAGAGTACAT34100.13062176342445062No Hit
GTACATGGGAAGCAGTGGTAAGCAG29810.11418870286460038No Hit
GTACTCTGCGTTGATACCACTGCTT29440.11277139927319141No Hit
GGTATCAACGCAGAGTACATGGGAA28060.10748523993226054No Hit
GCTTACCACTGCTTCCCATGTACTC27570.10560827031120538No Hit
ATGGTAAGCAGTGGTATCAACGCAG26810.10269705212344636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTC402.7640176E-416.62342314
ACGCACG604.1002696E-412.66425311
CGCACGA550.003073790812.08860412
TACGACC600.00589001411.0801594
GTATTAG3850.010.1312591
TACATCG856.613529E-410.05594355
GTCCTAA1803.8380676E-1010.0420271
TGGACCG1356.47231E-79.84903055
GTATTAA4150.09.6281191
GTCTAGC1606.495975E-89.5134991
CGATTTC900.00111403649.5000116
GTATAGG2451.8189894E-129.3193451
CTAATAC3100.09.1914443
GGACCGT1151.11429494E-49.0845296
TATACTG5350.09.0534825
TCTAGAC2106.712071E-109.0455483
CTAGACA2151.0477379E-98.8346794
CTCCTAG2502.5465852E-118.75241851
GTCTAAT2303.929017E-108.6862381
GTATAAT3750.08.6255731
Produced by FastQC (version 0.11.5)