FastQCFastQC Report
Wed 25 May 2016
SRR1294566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610591
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAAC121170.4641477734352107No Hit
CCCATGTACTCTGCGTTGATACCAC119290.45694633897075415No Hit
ACCATGTACTCTGCGTTGATACCAC107370.4112861800259022No Hit
GTGGTAAGCAGTGGTATCAACGCAG81290.3113854295828033No Hit
GTATCAACGCAGAGTACTTTTTTTT76600.2934201489241325No Hit
GGTAAGCAGTGGTATCAACGCAGAG65910.2524715667831537No Hit
CATGTACTCTGCGTTGATACCACTG61340.23496595215412908No Hit
GGTATCAACGCAGAGTACTTTTTTT51880.1987289468170234No Hit
CCATGTACTCTGCGTTGATACCACT49900.19114445732786178No Hit
GTGGTATCAACGCAGAGTACATGGG45930.17593717284706797No Hit
TATCAACGCAGAGTACTTTTTTTTT44970.17225984460989868No Hit
GAGTACATGGTAAGCAGTGGTATCA43820.16785471182578965No Hit
GCTTACCATGTACTCTGCGTTGATA40400.15475422998087407No Hit
ACTCTGCGTTGATACCACTGCTTAC39450.1511152072461753No Hit
GTATCAACGCAGAGTACATGGGAAG38300.14671007446206624No Hit
GCGTTGATACCACTGCTTACCATGT37130.14222833067301618No Hit
GCAGTGGTATCAACGCAGAGTACAT36770.14084933258407772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33100.1267912131773993No Hit
GTACATGGGAAGCAGTGGTAAGCAG31830.1219264143636441No Hit
GGTATCAACGCAGAGTACATGGGAA30980.11867044665365045No Hit
GTACTCTGCGTTGATACCACTGCTT30600.11721483755977094No Hit
ATGGTAAGCAGTGGTATCAACGCAG29040.11123917917437087No Hit
GCTTACCACTGCTTCCCATGTACTC27810.10652760237049771No Hit
GTATCAACGCAGAGTACATGGTAAG27100.10380791169509127No Hit
CTGCTTACCATGTACTCTGCGTTGA26750.10246721910862329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT350.002172003516.2842858
AACCGTC400.00528143414.2495697
CGACGTA400.005283447414.2487519
ACCGTTC550.003071423212.08984858
CTAGGGC1701.0913936E-1111.1778463
CCGTATA600.0058511911.0900091
GGCGACG600.00587879411.0829987
GAACCGT700.001490460510.8584796
CTAGCAC1701.382432E-1010.6189543
GTTCTAG1909.094947E-1210.5063231
CTTAGTA2350.010.1080132
TGCACCG856.588685E-410.0600615
TTGCGCT856.5979746E-410.0585197
AAGACCG951.6427551E-410.001235
TACACCG900.00111279519.5011685
CTATCCT2151.05501385E-109.28021054
GTGCTAG1651.05646905E-79.21766951
CTGTGCG2106.693881E-109.0468259
GTAGGAC2002.6520866E-99.026113
TAGCACC2002.6520866E-99.026114
Produced by FastQC (version 0.11.5)