FastQCFastQC Report
Wed 25 May 2016
SRR1294565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061361
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG14910.14048000633149324No Hit
ATTCCATTCCATTCCATTCCATTCC12880.12135362049293313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11790.11108378770277032No Hit
CCCATGTACTCTGCGTTGATACCAC11050.10411160764339372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG359.919405E-519.00412
AGTACGG551.1278473E-515.5451463
GTCCTAC550.0030365312.1072171
AGATAGC658.0305955E-411.6904226
CTAAACC752.0739227E-411.3992374
CTAATAC1104.963167E-711.227053
TAGTACC855.322568E-511.1757224
GTGTTAG1900.011.0148361
ATAGGAC1408.336428E-910.8569283
GTCTTAG1158.653078E-710.7536151
TCGTGGG803.775811E-410.68527412
ACAGTCC1355.6939825E-810.5533578
TGCACTG1659.276846E-1010.3624545
TCTGTCG750.00265096610.1312228
GTCTCAC1701.5188562E-910.0723911
CTTGCAC856.590474E-410.0586233
TAGAAGT1701.5570549E-910.0581494
TCTAAAC1151.0253965E-59.9128473
CAGGACC1151.0259711E-59.9123794
ACACCAT1856.475602E-109.7551846
Produced by FastQC (version 0.11.5)