FastQCFastQC Report
Wed 25 May 2016
SRR1294565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061361
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35700.3363605785401951No Hit
GGTATCAACGCAGAGTACTTTTTTT29320.27624908019043476No Hit
TATCAACGCAGAGTACTTTTTTTTT21370.2013452538768619No Hit
GTGGTATCAACGCAGAGTACATGGG14740.13887828929082566No Hit
ATTCCATTCCATTCCATTCCATTCC13630.12842001920176077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11620.10948207066210272No Hit
CCCATGTACTCTGCGTTGATACCAC11280.10627863658076751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG500.001488385513.3125481
GGCACGA600.00588595111.080215
GTTCTGG1408.210918E-910.8673861
GCAGGAC700.001487213410.8602143
GTAGGAC803.758715E-410.6905223
TCTAGAC1002.3889363E-510.4529553
GTCTAAG1105.970631E-610.3734141
CTTATAC1106.017861E-610.3665693
GGACTCC750.00264018610.1361996
GTATAAC856.532386E-410.0683131
CAAGACA1701.546141E-910.0616684
CTAGACT1151.0217151E-59.9158484
TAGACAG1356.406681E-79.8546385
GTACAAC1106.759076E-59.5089631
CTCTTAT1106.759076E-59.5089631
GTGCTAG1002.727033E-49.5089631
GTACTAG900.00110334189.5089631
GTCTTAC1002.727033E-49.5089631
CTATCCT1106.808077E-59.5026874
TAACCTG900.00111002519.50268655
Produced by FastQC (version 0.11.5)