FastQCFastQC Report
Wed 25 May 2016
SRR1294564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141634
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC53850.25144352396347835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA48780.22777001112234863No Hit
GTACATGGTAAGCAGTGGTATCAAC40820.19060212902858284No Hit
ACCATGTACTCTGCGTTGATACCAC38460.17958250569425027No Hit
GTGGTATCAACGCAGAGTACATGGG34570.16141880452028684No Hit
GTGGTAAGCAGTGGTATCAACGCAG33440.15614245944918692No Hit
CATGTACTCTGCGTTGATACCACTG25680.11990844373968661No Hit
GGTAAGCAGTGGTATCAACGCAGAG22880.10683431435997001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG350.00217469816.281079
TTCGCGG755.8609658E-815.19744114
GTACGGG456.6901377E-414.7991371
CGGGCGA801.2808232E-714.25758918
TCGCGGG801.2914643E-714.24793315
CTTCGCG905.42228E-712.66394213
GTTCTAA2200.011.6759411
CACGGGT855.3377546E-511.1738064
TGGCGTA855.3390242E-511.1735455
GACCGAT700.001495062510.85430057
CGCGGGC1158.829047E-710.73832416
CGGGTCT803.7810643E-410.6847036
GCGTACA909.543305E-510.5527927
GTATTAG2400.010.3065411
TAGGAGT1803.965397E-1010.0253874
CGTACAG951.64922E-49.99714858
AAGACCG1356.467799E-79.8492735
GTCTAAT1201.6719592E-59.513731
TCTTAGG1701.659646E-89.5019512
GGCGTAC1106.857539E-59.4975136
Produced by FastQC (version 0.11.5)