FastQCFastQC Report
Wed 25 May 2016
SRR1294564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141634
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73330.3424021097909353No Hit
CCCATGTACTCTGCGTTGATACCAC55560.25942808154894814No Hit
GGTATCAACGCAGAGTACTTTTTTT54720.2555058427350332No Hit
TATCAACGCAGAGTACTTTTTTTTT42510.1984932999756261No Hit
GTACATGGTAAGCAGTGGTATCAAC42380.19788628682585355No Hit
ACCATGTACTCTGCGTTGATACCAC41290.19279671503160672No Hit
GTGGTAAGCAGTGGTATCAACGCAG40050.1870067434491608No Hit
GTGGTATCAACGCAGAGTACATGGG37350.17439954726157691No Hit
CATGTACTCTGCGTTGATACCACTG27550.12864009443256877No Hit
GGTAAGCAGTGGTATCAACGCAGAG26160.12214972306192375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22370.10445295508009304No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22110.10323892878054794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG500.001497246313.3030915
ACGTTAT550.003049853512.1010671
ACCGGGG550.003072347712.08928
TCACCGG802.8737559E-511.8724916
AGACCGT1002.3927727E-510.4526736
GCTAGAT750.002642658310.1359253
GTCTTAC1606.0899765E-910.102231
TACTAGG1054.0925563E-59.9553912
TAAGACC2105.456968E-129.95492654
CTTACAC1856.4574124E-109.7592523
ACGTTGG900.00110535159.5079811
CTAAGAC1606.610571E-89.5024293
GCACCGC800.00450710959.5024296
ACCGGAG1201.705051E-59.49799218
GTATAGT1951.6570993E-99.2641871
ACTGTGC5150.09.2219978
TAAGACT2304.1836756E-119.089284
CCTATAC2201.6734703E-109.07053
CACCGGA1054.5027176E-49.04570717
TAGAACT2950.09.0192554
Produced by FastQC (version 0.11.5)