FastQCFastQC Report
Wed 25 May 2016
SRR1294563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103254
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC23030.20874612736504922No Hit
GTGGTATCAACGCAGAGTACATGGG20660.18726422020677017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20560.18635781062203263No Hit
GTACATGGTAAGCAGTGGTATCAAC20030.18155383982292383No Hit
ACCATGTACTCTGCGTTGATACCAC19060.17276166685096994No Hit
GTGGTAAGCAGTGGTATCAACGCAG17230.15617437145027346No Hit
GTATCAACGCAGAGTACTTTTTTTT15490.14040284467584074No Hit
CATGTACTCTGCGTTGATACCACTG11560.10478094799565647No Hit
GGTATCAACGCAGAGTACTTTTTTT11520.10441838416176147No Hit
GGTAAGCAGTGGTATCAACGCAGAG11040.10006761815502141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT307.7283476E-418.99723414
CGGTGAA707.275703E-613.5682239
GTGTAGG1251.378794E-912.1758941
GGTTAGA951.0225885E-612.0156841
ACACGGT802.8701943E-511.8727336
GGCTAGG657.932763E-411.7075911
ACGGTGA907.470815E-611.6088958
CTTATGC752.0773271E-411.3973073
GTACTGC700.00147464810.8713341
AACACGG700.001493183510.8550715
CTAGGAC803.7770395E-410.6849763
CTAGACA1553.6652636E-910.4173654
GTATAAC1105.9454887E-610.3771821
CAGGACT1502.4469045E-810.1313994
AGAACTG1606.177288E-910.0918245
GTATAGC951.6213242E-410.013071
GTCTTAT1054.0428167E-59.9653891
CTGTCGC1054.1119314E-59.950039
TCCGCCT1151.0281556E-59.9107029
ATACAGC1752.57387E-99.7695636
Produced by FastQC (version 0.11.5)