FastQCFastQC Report
Wed 25 May 2016
SRR1294563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103254
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56110.5085864179962185No Hit
GGTATCAACGCAGAGTACTTTTTTT45670.41395725734962213No Hit
TATCAACGCAGAGTACTTTTTTTTT34380.3116236152327569No Hit
CCCATGTACTCTGCGTTGATACCAC25170.22814329247843199No Hit
GTGGTATCAACGCAGAGTACATGGG22000.19941010864225284No Hit
GTACATGGTAAGCAGTGGTATCAAC21150.19170562717198397No Hit
ACCATGTACTCTGCGTTGATACCAC19410.17593410039755125No Hit
GTGGTAAGCAGTGGTATCAACGCAG18710.16958923330438866No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17670.1601625736231185No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15110.1369584882538382No Hit
CATGTACTCTGCGTTGATACCACTG13290.12046183381161546No Hit
GGTAAGCAGTGGTATCAACGCAGAG12530.11357312096761035No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11730.10632184428971025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT701.0921941E-412.21207112
ACCGTGT658.0293353E-411.6907188
GTTCTAG855.2777665E-511.185091
TTAGCAC1355.6474164E-810.5593853
GTCTTGC1909.094947E-1210.5080991
TAGACTG1553.6325218E-910.4231355
TAGACAG1659.2040864E-1010.3673965
TTACACC750.00263857710.137014
GACGGGG750.002650513810.13149611
TGGACTG951.6368374E-410.0036295
CTAGTAC951.6368374E-410.0036293
CTAGGAC1054.0849583E-59.9559923
AGACTGT1651.0135409E-89.79142956
TTAGTAG1651.0135409E-89.79142953
GCATAAG1106.772463E-59.5073271
GTCTAGG1201.6829848E-59.5073271
GTGCTAT1606.542359E-89.5073271
GTATAAA2303.6379788E-129.5073271
GGTTAAG900.00110515189.5073271
GTCTAAA1002.7319696E-49.5073261
Produced by FastQC (version 0.11.5)