FastQCFastQC Report
Wed 25 May 2016
SRR1294562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418395
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC31100.2192619122317831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA29220.2060074943862605No Hit
GTGGTATCAACGCAGAGTACATGGG27020.1904970054180958No Hit
GTACATGGTAAGCAGTGGTATCAAC25040.17653756534674755No Hit
ACCATGTACTCTGCGTTGATACCAC24260.17103839198530732No Hit
GTATCAACGCAGAGTACTTTTTTTT22120.155950916352638No Hit
GTGGTAAGCAGTGGTATCAACGCAG18710.1319096584519827No Hit
GGTATCAACGCAGAGTACTTTTTTT16010.11287405835468964No Hit
CATGTACTCTGCGTTGATACCACTG14610.10300374719313025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC853.90288E-612.3052631
CCGTGTG752.0785445E-411.397069
GTACTAG700.001480030810.8669851
GTAGGAC1053.4727018E-610.8551073
TAGACAG2300.010.7371175
GTAATAC1553.6707206E-910.4174023
CCAATAC1201.5265359E-610.2897393
GTATTAG2250.010.142521
TAGTACA1901.0004442E-109.9981264
GTGTAGC1054.0628613E-59.9614031
ATTAGAC1451.6201193E-79.8257453
TTAAGAC1651.024091E-89.7860443
TGGACTG2054.1836756E-119.7298835
GTATTGG1401.0447438E-69.5086131
GTCTTAC1502.6111593E-79.5086131
CCTAGAC1002.7596453E-49.49821953
CTAAGCT1106.847568E-59.49821954
ACTAGAC1002.7596453E-49.49821953
GTAGAAC800.0045220259.49821953
CTAGACA2106.730261E-119.4982194
Produced by FastQC (version 0.11.5)