FastQCFastQC Report
Wed 25 May 2016
SRR1294562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418395
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76620.5401880294276278No Hit
GGTATCAACGCAGAGTACTTTTTTT53740.3788789441587146No Hit
TATCAACGCAGAGTACTTTTTTTTT45760.3226181705378262No Hit
CCCATGTACTCTGCGTTGATACCAC32050.22595962337712697No Hit
GTGGTATCAACGCAGAGTACATGGG29010.20452694771202662No Hit
GTACATGGTAAGCAGTGGTATCAAC26440.18640787650830692No Hit
ACCATGTACTCTGCGTTGATACCAC25530.17999217425329334No Hit
GTGGTAAGCAGTGGTATCAACGCAG23570.16617373862711024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23410.16504570306578914No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20380.14368352962327138No Hit
GGTAAGCAGTGGTATCAACGCAGAG16740.11802072060321703No Hit
CATGTACTCTGCGTTGATACCACTG16220.11435460502892353No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16090.11343807613535016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG250.006027430819.0023985
GTCTTAC951.8189894E-1116.0076641
ACCGCGC604.0945815E-412.665585518
GTCTAGC951.0335534E-612.0057481
TAGGACT1900.011.0013894
CCGCGCC700.001492325710.856215519
CACCGCG700.001492764710.85583317
GTCTAGG1355.6434146E-810.5606111
TAGACAG1803.45608E-1110.5568885
GGACCAT1106.033757E-610.36494356
CTAGGAC1201.5204077E-610.2929663
CTAGCAC1303.8322105E-710.232063
GAGTACG856.563229E-410.0636421
GTCCTAT951.6356692E-410.0047891
TAAGGGT1151.0243981E-59.9142954
TTATACT2400.09.8970824
TATACTG3450.09.6388985
ATTAGGC800.00451034039.5011993
AGGACTG2201.6370905E-119.5011995
TAGCACA1502.640918E-79.5011994
Produced by FastQC (version 0.11.5)