FastQCFastQC Report
Wed 25 May 2016
SRR1294561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379178
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC126440.5314440533663307No Hit
ACCATGTACTCTGCGTTGATACCAC82270.3457916978048721No Hit
GTACATGGTAAGCAGTGGTATCAAC79380.3336446453354898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA76190.3202366531634035No Hit
GTGGTAAGCAGTGGTATCAACGCAG73950.31082163671654667No Hit
CATGTACTCTGCGTTGATACCACTG65910.2770284526840783No Hit
GGTAAGCAGTGGTATCAACGCAGAG54450.2288605560407838No Hit
GTGGTATCAACGCAGAGTACATGGG46500.1954456539191267No Hit
GTATCAACGCAGAGTACATGGGAAG41710.17531264999928547No Hit
CCATGTACTCTGCGTTGATACCACT39360.16543528899477047No Hit
ACTCTGCGTTGATACCACTGCTTAC36670.15412886299385756No Hit
GTACATGATAAGCAGTGGTATCAAC34540.14517619110465885No Hit
GCAGTGGTATCAACGCAGAGTACAT34400.1445877525767303No Hit
GGTATCAACGCAGAGTACATGGGAA32950.1384932106803274No Hit
GTACATGGGAAGCAGTGGTAAGCAG32950.1384932106803274No Hit
ATCATGTACTCTGCGTTGATACCAC31540.1325667940776184No Hit
GTACTCTGCGTTGATACCACTGCTT30760.1292883508505879No Hit
GCTTACCACTGCTTCCCATGTACTC30010.1261360015938278No Hit
GAGTACATGGTAAGCAGTGGTATCA29910.1257156883595931No Hit
GCTTACCATGTACTCTGCGTTGATA29200.12273146439652687No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA27400.11516582618030262No Hit
TATCAACGCAGAGTACATGGGAAGC25450.10696971811272632No Hit
GTACATGGGAAGCAGTGGTATCAAC24780.10415361944335397No Hit
AAGCAGTGGTATCAACGCAGAGTAC24640.10356518091542541No Hit
GTATCAACGCAGAGTACTTTTTTTT24330.1022622098892979No Hit
GCGTTGATACCACTGCTTACCATGT24120.10137955209740507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG801.9971212E-613.0645682
AGGCGTC550.003075787612.0874847
CTCGGTC1103.8333383E-812.0872315
TCGGTCT907.490431E-611.6075796
CCTCGGT1553.6816346E-910.4164044
GTTCTAG2200.010.3785911
TAGCACT3150.010.2510654
AGCCTCG2400.09.89742
GTACTAG1304.1631174E-69.5137081
TCGTGGG1606.66314E-89.49770812
CTAGACT1804.2055035E-99.497314
GTAATAC1951.6916601E-99.2539833
TTAGAAC1752.6822818E-89.2261513
CGGTCTC1252.7641338E-59.1174177
GGTCTAC1151.09363405E-49.1000681
TCTATAC2304.1836756E-119.0845743
CACAACG1151.1143793E-49.0843829
TTAGACT2002.6702764E-99.0224444
GTCTCGT950.00182890688.9974519
GGACCAT1804.2409738E-88.9694936
Produced by FastQC (version 0.11.5)