FastQCFastQC Report
Wed 25 May 2016
SRR1294561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379178
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC134740.5663300518078093No Hit
GTATCAACGCAGAGTACTTTTTTTT85980.3613853187949788No Hit
ACCATGTACTCTGCGTTGATACCAC85580.35970406585804005No Hit
GTGGTAAGCAGTGGTATCAACGCAG82610.34722076280127No Hit
GTACATGGTAAGCAGTGGTATCAAC79480.33406495856972446No Hit
CATGTACTCTGCGTTGATACCACTG70910.29804411439581235No Hit
GGTAAGCAGTGGTATCAACGCAGAG59120.24848918407954346No Hit
GGTATCAACGCAGAGTACTTTTTTT58630.2464296492317935No Hit
TATCAACGCAGAGTACTTTTTTTTT52080.2188991323894219No Hit
GTGGTATCAACGCAGAGTACATGGG48870.20540707757048865No Hit
GTATCAACGCAGAGTACATGGGAAG44370.18649298202992798No Hit
CCATGTACTCTGCGTTGATACCACT41490.17438796088396916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39660.1666962286974745No Hit
ACTCTGCGTTGATACCACTGCTTAC39610.16648607208035715No Hit
GCAGTGGTATCAACGCAGAGTACAT37670.15833199533620435No Hit
GGTATCAACGCAGAGTACATGGGAA35800.15047213785601582No Hit
GTACATGATAAGCAGTGGTATCAAC35580.1495474487406995No Hit
GTACATGGGAAGCAGTGGTAAGCAG34490.14496603448754147No Hit
ATCATGTACTCTGCGTTGATACCAC32770.137736646858705No Hit
GAGTACATGGTAAGCAGTGGTATCA31410.13202038687311332No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31290.13151601099203172No Hit
GCTTACCACTGCTTCCCATGTACTC30610.1286578809992359No Hit
GCTTACCATGTACTCTGCGTTGATA30130.12664037747490942No Hit
GTACTCTGCGTTGATACCACTGCTT29920.12575771968301655No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28260.11878051999472086No Hit
AAGCAGTGGTATCAACGCAGAGTAC27690.11638473455958319No Hit
TATCAACGCAGAGTACATGGGAAGC26570.11167722633615476No Hit
GTACATGGGAAGCAGTGGTATCAAC26420.11104675648480272No Hit
GCGTTGATACCACTGCTTACCATGT25900.10886112766678241No Hit
CAGTGGTATCAACGCAGAGTACATG24180.10163174003794588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1301.9645086E-1012.4317831
GGACCGT701.0902317E-412.21565156
ATAAGAC1701.382432E-1010.619283
TATACTG3950.010.3429285
TTAGTAC2500.010.2615783
GTCTTAG2151.8189894E-1210.1699131
ACAGCGT856.59783E-410.0584688
TACTAGG1151.02423E-59.9151942
ATTAGAC1606.6227585E-89.5014613
TTAGACT2002.6375346E-109.5014614
GACAGTA1606.635673E-89.5002637
CTGCGTA800.00451825479.4996639
TAGTACT3150.09.3506444
TAGACAG2650.09.3217965
GTCCTAG1856.6211214E-99.2497211
CCTAAAC1752.6640919E-89.2299913
CTAATAC1752.6640919E-89.2299913
CTATACT3400.09.2220064
ACTAGAC1651.0635631E-79.2135383
CAGGACC3650.09.1109914
Produced by FastQC (version 0.11.5)