FastQCFastQC Report
Wed 25 May 2016
SRR1294560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743855
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC108170.39422637129148586No Hit
ACCATGTACTCTGCGTTGATACCAC87120.31750948938628315No Hit
GTACATGGTAAGCAGTGGTATCAAC83950.30595640075732866No Hit
GTGGTAAGCAGTGGTATCAACGCAG63170.23022353586468672No Hit
CATGTACTCTGCGTTGATACCACTG58820.21436992844009614No Hit
GGTAAGCAGTGGTATCAACGCAGAG49300.1796742174786933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49280.17960132732961473No Hit
GTGGTATCAACGCAGAGTACATGGG47740.17398878585056426No Hit
CCATGTACTCTGCGTTGATACCACT39440.14373937398295464No Hit
GTATCAACGCAGAGTACATGGGAAG38070.1387463987710721No Hit
ATTCCATTCCATTCCATTCCATTCC35970.13109293311782147No Hit
ACTCTGCGTTGATACCACTGCTTAC35640.12989024565802493No Hit
GTACATGGGAAGCAGTGGTAAGCAG32450.11826426687999185No Hit
GAGTACATGGTAAGCAGTGGTATCA31130.11345351704080574No Hit
GCAGTGGTATCAACGCAGAGTACAT31000.11297973107179497No Hit
GCTTACCACTGCTTCCCATGTACTC30240.11020990540680903No Hit
GAATGGAATGGAATGGAATGGAATG30220.11013701525773045No Hit
GCTTACCATGTACTCTGCGTTGATA30210.11010057018319118No Hit
GGTATCAACGCAGAGTACATGGGAA29990.10929877854332683No Hit
GTACTCTGCGTTGATACCACTGCTT29770.10849698690346248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAG350.002154264416.3043331
CGCACGA400.00528965114.24626712
TAAGACG400.00528965114.2462674
CGTAGAC500.001503020813.2965183
TTGAACG550.003075501212.0877434
GTCTAAG2200.011.24010851
TGTACCG600.005889868411.0802295
GTATTAG3100.010.7380691
CGGAGCG803.7818003E-410.68473
GTACCGT803.7830745E-410.6843116
GTACTAG1701.364242E-1010.6297851
GTCCTAT1803.45608E-1110.5676221
CAGGACG909.5455645E-510.5527914
AAATGCG1106.069762E-610.3607345
TCCGTAC1106.073724E-610.3601678
GTCTAAA2450.010.0931591
AAGGCGA856.614492E-410.0558226
CGGAATA1252.5802983E-69.8774134
GTAGCAC2850.09.6641353
TATACTA2850.09.6639595
Produced by FastQC (version 0.11.5)