FastQCFastQC Report
Wed 25 May 2016
SRR1294560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743855
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC118910.433368381346682No Hit
ACCATGTACTCTGCGTTGATACCAC89330.325563850859466No Hit
GTACATGGTAAGCAGTGGTATCAAC86630.315723680733858No Hit
GTATCAACGCAGAGTACTTTTTTTT86150.3139743171559722No Hit
GTGGTAAGCAGTGGTATCAACGCAG69440.25307459760082074No Hit
CATGTACTCTGCGTTGATACCACTG61660.22472032960925412No Hit
GGTATCAACGCAGAGTACTTTTTTT59080.21531750037811764No Hit
GGTAAGCAGTGGTATCAACGCAGAG53180.19381490639993731No Hit
TATCAACGCAGAGTACTTTTTTTTT52130.189988173573312No Hit
GTGGTATCAACGCAGAGTACATGGG48460.17661283121739305No Hit
CCATGTACTCTGCGTTGATACCACT42320.1542355554502698No Hit
GTATCAACGCAGAGTACATGGGAAG39730.144796281144594No Hit
ACTCTGCGTTGATACCACTGCTTAC39160.14271891189585456No Hit
ATTCCATTCCATTCCATTCCATTCC36970.13473744057175033No Hit
GCAGTGGTATCAACGCAGAGTACAT33040.12041452627780988No Hit
GTACATGGGAAGCAGTGGTAAGCAG32380.11800915135821682No Hit
GAGTACATGGTAAGCAGTGGTATCA31920.11633267792940953No Hit
GCTTACCATGTACTCTGCGTTGATA31690.11549444121500589No Hit
GGTATCAACGCAGAGTACATGGGAA31500.11480198479875942No Hit
GCTTACCACTGCTTCCCATGTACTC30330.11053791107766263No Hit
GAATGGAATGGAATGGAATGGAATG29070.10594583168571224No Hit
GTACTCTGCGTTGATACCACTGCTT28810.10499825974769074No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28230.10288444542441201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC700.00148968210.8591953
TGTACCG700.001489908610.8589975
GTCCTAC1701.382432E-1010.6239151
TCTAGAC1803.45608E-1110.557553
GACTGCG856.595961E-410.0588917
TATTCCG1054.0996223E-59.9540815
CTAGTAC1554.055073E-89.8083063
GTATTAA5350.09.77211951
CTAGGAC2054.1836756E-119.7335473
TAGTACT2350.09.7039614
GTCCTAG2201.6370905E-119.5056081
GGTCTAG1606.580376E-89.5056081
CGGAACA800.00451027239.5017964
CTTACAC3700.09.5017953
TAACCCG1502.643592E-79.5016225
GTATAGA2850.09.3388421
CTTAGAC1651.06330845E-79.2138613
GTTCTAG2600.09.1400081
TCTACAC2750.08.9835173
ATAGGAC1701.6811464E-78.9428663
Produced by FastQC (version 0.11.5)